Research Article | Volume: 8, Issue: 9, September, 2018

Analysis of vitexin in aqueous extracts and commercial products of Andean Passiflora species by UHPLC-DAD

Paula Sepúlveda Geison M. Costa Diana Marcela Aragón Freddy Ramos Leonardo Castellanos   

Open Access   

Published:  Sep 30, 2018

DOI: 10.7324/JAPS.2018.8912
Abstract

Plants of the Passiflora genus are extensively cultivated in South America, as their edible fruits are widely commercialized. They are also recognized worldwide for their ethnopharmacological uses. Different Pharmacopoeias indicate vitexin as the chemical marker for P. incarnata, the most widely studied Passiflora species worldwide. In the present work, some species of Passiflora from the Andean region of Colombia and commercial phytotherapeutic products were evaluated for their vitexin content by UHPLC-DAD. From the studied species: ‘banana passion fruits’ (P. tripartita var tripartita, P. tripartita var mollissima, P. mixta, P. cumbalensis, P. tarminiana), ‘passion fruits’ (P. edulis var flavicarpa, P. edulis var edulis), ‘granadillas’, (P. ligularis, P. quadrangularis) and ‘sweet passion fruit’ (P. alata), the aqueous extracts of P. mixta, P. tripartita var mollissima and P. edulis var edulis showed quantifiable amounts of vitexin (4.58 ± 1.23; 2.49 ± 0.2; 0.3 ± 0.0 mg g−1 dry extract, respectively). Additionally, four of the six botanical phytotherapeutic products tested showed considerable quantities of this flavonoid. The results obtained suggest that vitexin cannot be used as the only chemical marker for the quality control of the studied Passiflora species.


Keyword:     PassifloravitexinflavonoidsUHPLCquality control.


Citation:

Sepúlveda P, Costa GM, Aragón DM, Ramos F, Castellanos L. Analysis of vitexin in aqueous extracts and commercial products of Andean Passiflora species by UHPLC-DAD. J App Pharm Sci, 2018; 8(09): 081-086.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Agência Nacional de Vigilância Sanitária, Fundação Oswaldo Cruz. 2010. Farmacopeia Brasileira. Brasília, Brasil.

Ayres A, Araújo L, Soares TC, Costa GM, Reginatto FH, Ramos FA, Castellanos L, Schenkel EP, Soares VP, Zucolotto SM, Gavioli EC. Comparative central effects of the aqueous leaf extract of two populations of Passiflora edulis. Rev Bras Farmacogn, 2015; 25(5):499-505. https://doi.org/10.1016/j.bjp.2015.06.007

British Pharmacopoeia. 2009. Passion Flower. London, England.

Cardozo RH, Córdoba SL, González JD, Guzman JR, Lancheros HO, Mesa LI, Pacheco RA, Pérez BA, Ramos FA, Torres ME, Zú-iga PT. 2009. Especies útiles en la Región Andina de Colombia Tomo I. Bogotá, Colombia. Ed. Jardín Botánico de Bogotá José Celestino Mutis.

Chen F, Zhang Q, Liu J, Gu H, Yang L. An efficient approach for the extraction of orientin and vitexin from Trollius chinensis flowers using ultrasonic circulating technique. Ultrason Sonochem, 2017; 37:267-278. https://doi.org/10.1016/j.ultsonch.2017.01.012

Costa GM, Gazola AC, Madóglio FA, Zucolotto SM, Reginatto FH, Castellanos L, Ramos FA, Duque C, Schenkel EP. Vitexin derivatives as chemical markers in the differentiation of the closely related species Passiflora alata Curtis and Passiflora quadrangularis Linn. J Liq Chromatogr Relat Technol, 2013; 36:1697-707.

Costa GM, Gazola AC, Zucolotto SM, Castellanos L, Ramos FA, Reginatto HF, Schenkel EP. Chemical profiles of traditional preparations of four South American Passiflora species by chromatographic and capillary electrophoretic techniques. Rev Bras Farmacogn, 2016; 26(4):451-8. https://doi.org/10.1016/j.bjp.2016.02.005

Dhawan K, Dhawan S, Sharma A. Passiflora: A review update. J Ethnopharmacol, 2004; 94(1):1-23. https://doi.org/10.1016/j.jep.2004.02.023

European Pharmacopoeia. 2011. Passion Flower.

Farabegoli F, Scarpa ES, Frati A, Serafini G, Papi A, Spisni E, Antonini E, Benedetti S, Ninfali P. Betalains increase vitexin-2-O-xyloside cytotoxicity in CaCo-2 cancer cells. Food Chem, 2017; 218:356-64. https://doi.org/10.1016/j.foodchem.2016.09.112

Farag MA, Otify A, Porzel A, Michel CG, Elsayed A, Wessjohann LA. 2016. Comparative metabolite profiling and fingerprinting of genus Passiflora leaves using a multiplex approach of UPLC-MS and NMR analyzed by chemometric tools. Springer.

Fischer IH, Rezende JA. Diseases of Passion Flower (Passiflora spp). Pest Technol, 2008; 2(1):1-19.

Gomes SVF, Portugal LA, dos Anjos JP, de Jesus ON, de Oliveira EJ, David JP, David JM. Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species. Microchem J, 2017; 132:28-35. https://doi.org/10.1016/j.microc.2016.12.021

Hernández R, Bernal A. Lista de Especies de Passifloraceae de Colombia. Biota Colomb, 2000; 1(3):320-35.

He M, Min JW, Kong WL, He XH, Li JX, Peng BW. A review on the pharmacological effects of vitexin and isovitexin. Fitoterapia, 2016; 115:74-85. https://doi.org/10.1016/j.fitote.2016.09.011

ICH. 2005. Validation of Analytical Procedures: Text and Methodology - Q2(R1). London, England.

INVIMA. Instituto Nacional de Vigilancia de Medicamentos y Alimentos. 2017. Consulta de registros sanitarios. [ONLINE] Available at: http://farmacovigilancia.invima.gov.co:8082/Consultas/consultas/consreg_ encabcum.jsp. [Accessed 4 Mar 2017].

Li H, Zhou P, Yang Q, Shen Y, Deng J, Li L, Zhao D. Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis "edulis" and Passiflora edulis "flavicarpa". J Ethnopharmacol, 2011; 133(3):1085-90. https://doi.org/10.1016/j.jep.2010.11.039

Ninfali P, Bacchiocca M, Antonelli A, Biagiotti E, Di Gioacchino AM, Piccoli G, Stocchi V, Brandi G. Characterization and biological activity of the main flavonoids from Swiss Chard (Beta vulgaris subspecies cycla). Phytomedicine, 2007; 14(2-3):216-21. https://doi.org/10.1016/j.phymed.2006.03.006

Ocampo J, Coppens G, Restrepo M, Jarvis A, Salazar M, Caetano C. Diversity of Colombian Passifloraceae: biogeography and an updated list for conservation. Biota Colomb, 2007; 8(1):1-45.

Ocampo J, Wyckhuys K. 2012. Tecnología para el cultivo de la Gulupa (Passiflora edulis f. edulis Sims) en Colombia. Bogotá, Colombia: Centro de Bio-Sistemas de la Universidad Jorge Tadeo Lozano, Centro Internacional de Agricultura Tropical - CIAT y Ministerio de Agricultura y Desarrollo Rural.

Patel SS, Soni H, Mishra K, Singhai AK. Recent updates on the genus Passiflora: A review. Int J Res Phytochem Pharmacol, 2011; 1(1):1- 16.

Ramos FA, Castellanos L, López C, Palacios L, Duque C, Pacheco R, Guzmán A. An Orientin Derivative Isolated from Passiflora tripartita var. mollissima. Latin American Journal of Pharmacy, 2010; 29(1):141-3.

Real Farmacopea Espa-ola. 2002. Passiflora, Sumidad De Passiflorae herba.

Scarpa ES, Antonini E, Palma F, Mari M, Ninfali P. Antiproliferative activity of vitexin-2-O-xyloside and avenanthramides on CaCo-2 and HepG2 cancer cells occurs through apoptosis induction and reduction of pro-survival mechanisms. Eur J Nutr, 2017; 1-15.

Wei W, Ying X, Zhang W, Chen Y, Leng A, Jiang C, Liu J. Effects of vitexin-2"-O-rhamnoside and vitexin-4"-O-glucoside on growth and oxidative stress-induced cell apoptosis of human adipose-derived stem cells. J Pharm Pharmacol, 2014; 66(7):988-97. https://doi.org/10.1111/jphp.12225

Zucolotto SM, Fagundes C, Reginatto FH, Ramos FA, Castellanos L, Duque C, Schenkel EP. Analysis of C-glycosyl flavonoids from South American Passiflora species by HPLC-DAD and HPLC-MS. Phytochem Anal, 2012; 23(3):232-9. https://doi.org/10.1002/pca.1348

Article Metrics
724 Views 93 Downloads 817 Total

Year

Month

Related Search

By author names