Development and Validation Method for Simultaneous Analysis of Retinoic Acid, Hydroquinone and Corticosteroid in Cream Formula by High-Performance Liquid Chromatography

Elvi Rahmayuni; Harmita Harmita; Herman Suryadi

doi:10.7324/JAPS.2018.8913

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Abstract

A simple, precise and rapid reverse phase HPLC-PDA method has been developed and validated for the simultaneous analysis of hydroquinone (HYQ), dexamethasone (DEX), triamcinolone acetonide (TSA), hydrocortisone acetate (HYA), betamethasone valerate (BEV) and retinoic acid (REA) in the cream dosage form. The mixture of HYQ, DEX, TSA, HYA, BEV and REA was separated on Waters X Bridge C18 5 μm column (4.6 mm × 250 mm). All separations were performed with a 2998 Photodiode Array (PDA) detector on 210-400 nm wavelength, 400C at column temperature and flow rate at 1.2 ml/min. The mobile phase was acetonitrile (ACN): 0.1% formic acid with gradient system. The retention times of HYQ, DEX, TSA, HYA, BEV, and REA were found to be 3.69; 9.13; 9.47; 9.96; 14.92 and 21.20. The method showed good linearity with correlation coefficient 0.9990; 0.9991; 0.9984; 0.9991; 0.9997 and 0.9991 over the range of 25-150 μg/ml for HYQ, DEX, TSA, HYA, BEV and REA, respectively. The method was mean recoveries in the range of 99.05 to 100.96% for all analytes. The developed method can be used in the routine analysis of HYQ, DEX, TSA, HYA, BEV and REA in the cream formula, as well as for qualitative interest in cosmetic preparations or for quantitative interest in drug preparations.

Keyword: Cream Hydroquinone Retinoic acid Corticosteroid Validation HPLC.

Citation:

Rahmayuni E, Harmita H, Suryadi H. Development and Validation Method for Simultaneous Analysis of Retinoic Acid, Hydroquinone and Corticosteroid in Cream Formula by HighPerformance Liquid Chromatography. J App Pharm Sci, 2018; 8(09): 087-092.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reference

Asean Cosmetic Method. Identification and Determination of Hydroquinone in Cosmetic Products by TLC and HPLC. 2005. ACM INO 03.
Asean Cosmetic Method. Identification of hydrocortisone Acetate, dexamethasone, betamethasone, betamethasone 17 valerate and triamcinolone acetonide in cosmetic products by TLC and HPLC. 2005. ACM MAL 07.
Asean Cosmetic Method. Identification of Retinoic acid (Retinoic Acid) in Cosmetic Products by TLC and HPLC. 2005. ACM 001.
Aung Myo Htet, EI Ei Thin, Mi Mi Saw, Soe Win. Chemical analysis of hydroquinone and retinoic acid contents in facial whitening creams. Asian Journal Of Pharmaceutical Sciences II. 2016. pp. 89-90.
Charlene DeHaven. Hydroquinone. www.innovativeskincare. 2014.
Desmedt B, Van Hoeck E, Rogiers V, Courselle P, De Beer JO, De Paepe K, Deconinck E. Characterisation of suspected illegal skin whitening cosmetics. J Pharm Biomed Anal, 2014; 90:85-91.https://doi.org/10.1016/j.jpba.2013.11.024
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Gimeno P, Maggio AF, Bancilhon M, Lassu N, Gornes H, Brenier C, Lempereur L. HPLC-UV Method for the identification and screening of Hydroquinone, ethers of hydroquinone and corticosteroids possibly used as skin-whitening agents in Illicit Cosmetic Products. Journal of Chromatographic Science, 2016; 54(3):343-352.
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Sheliya K., Shah K., Kapupura P. Development and validation of analytical method for simultaneous estimation of mometasone furoate, hydroquinone and retinoic acid in the topical formulation by RP-HPLC. Journal of Chemical and Pharmaceutical Research, 2014; 6(4):934-940.
United States Pharmacopoeia (40nd ed.). Rockville: The United States Pharmacopeial Convention. 2016.

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Asean Cosmetic Method. Identification and Determination of Hydroquinone in Cosmetic Products by TLC and HPLC. 2005. ACM INO 03.

Asean Cosmetic Method. Identification of hydrocortisone Acetate, dexamethasone, betamethasone, betamethasone 17 valerate and triamcinolone acetonide in cosmetic products by TLC and HPLC. 2005. ACM MAL 07.

Asean Cosmetic Method. Identification of Retinoic acid (Retinoic Acid) in Cosmetic Products by TLC and HPLC. 2005. ACM 001.

Aung Myo Htet, EI Ei Thin, Mi Mi Saw, Soe Win. Chemical analysis of hydroquinone and retinoic acid contents in facial whitening creams. Asian Journal Of Pharmaceutical Sciences II. 2016. pp. 89-90.

Charlene DeHaven. Hydroquinone. www.innovativeskincare. 2014.

Desmedt B, Van Hoeck E, Rogiers V, Courselle P, De Beer JO, De Paepe K, Deconinck E. Characterisation of suspected illegal skin whitening cosmetics. J Pharm Biomed Anal, 2014; 90:85-91.https://doi.org/10.1016/j.jpba.2013.11.024

Deswal A, Dhanda H. Pharmacology, and impact of retinoic acid on the treatment of premalignant squamous lesions of the oral cavity. The Pharma Innovation Journal, 2017; 6(7):20-26.

Gimeno P, Maggio AF, Bancilhon M, Lassu N, Gornes H, Brenier C, Lempereur L. HPLC-UV Method for the identification and screening of Hydroquinone, ethers of hydroquinone and corticosteroids possibly used as skin-whitening agents in Illicit Cosmetic Products. Journal of Chromatographic Science, 2016; 54(3):343-352.

Laurence Brunton, Bruce Chabner, Bjorn Knollman. Goodman & Gilman's the pharmacological basis of therapeutics (12th ed.). New York: McGraw-Hill Companies Inc. 2011.

Petkova N., P. Brabant, An. Masson, P. Denev. 2013. HPLC analysis of mono- and disaccharides in food products, Scientific works of UFT, Volume LX, "Food Science, engineering and technologies", 18-19 October 2013, Plovdiv, 761-765.

Sheliya K., Shah K., Kapupura P. Development and validation of analytical method for simultaneous estimation of mometasone furoate, hydroquinone and retinoic acid in the topical formulation by RP-HPLC. Journal of Chemical and Pharmaceutical Research, 2014; 6(4):934-940.

United States Pharmacopoeia (40nd ed.). Rockville: The United States Pharmacopeial Convention. 2016.