Research Article | Volume: 8, Issue: 9, September, 2018

Isolation, Identification and Medium Optimization for Tyrosinase Production by a Newly Isolated Bacillus subtilis NA2 Strain

Elsayed Ahmed Elsayed ,  Enas Nabil Danial   
Elsayed Ahmed Elsayed1 & 3, Enas Nabil Danial2 & 3

1Bioproducts Research Chair, Zoology Department, Faculty of Science, King Saud University, 11451 Riyadh, Kingdom of Saudi Arabia.

2Applied Biochemistry Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia.

3Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Cairo, Egypt.

Open Access   

Published:  Sep 30, 2018

DOI: 10.7324/JAPS.2018.8914
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Abstract

Tyrosinase (EC 1.14.18.1, monophenol, O-diphenol: oxygen oxidoreductase) is a copper-metallo-bifunctional enzyme, which catalyzes the O-hydroxylation and oxidation of monophenols to quinones. Tyrosinase plays an important role in the immune defense mechanisms of many prokaryotic and eukaryotic organisms. It also finds many applications in antioxidants and therapeutic fields. In the present work, a newly isolated tyrosinase-producing strain (Bacillus subtilis NA2) was identified using 16S rDNA and BLAST analysis. The primary cultivation medium produced tyrosinase at 416.67 U/min/mL with a yield coefficient (YP/X) of 111.75 × 103 U/g cells. Furthermore, the cultivation medium for the production of tyrosinase was optimized. The final optimized cultivation medium composed of (g/L): sucrose, 10; KNO3, 10; K2HPO4, 0.5; MgSO4.7H2O, 0.25; tyrosine, 10 and the initial optimized pH of the cultivation medium was 8.5. It has been shown, that the final optimized medium with the optimized pH resulted in an increase of about 39% in tyrosinase production from the initial un-optimized conditions. The new tyrosinase producing strain was identified and its volumetric production was significantly increased after the successful optimization of the production medium.


Keyword:     Isolation Identification Bacillus Tyrosinase Production Yield coefficient.

Citation:

Elsayed EA, Danial EN. Isolation, Identification and Medium Optimization for Tyrosinase Production by a Newly Isolated Bacillus subtilis NA2 Strain. J App Pharm Sci, 2018; 8(09): 093- 101.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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