Research Article | Volume: 5, Issue: 11, November, 2015

Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob.

Mariquit M. De Los Reyes ,  Glenn G. Oyong,  Virgilio D. Ebajo Jr.,  Vincent Antonio S. Ng,  Chien-Chang Shen,  Consolacion Y. Ragasa   
Mariquit M. De Los Reyes1 & 2, Glenn G. Oyong3, Virgilio D. Ebajo Jr.4, Vincent Antonio S. Ng4, Chien-Chang Shen5, Consolacion Y. Ragasa4 & 6

1Biology Department, De La Salle University Science & Technology Complex, Leandro V. Locsin Campus, Biñan City, Laguna 4024, Philippines.

2Biology Department, De La Salle University, 2401 Taft Avenue, Manila 0922, Philippines.

3Center for Natural Science and Ecological Research, De La Salle University, 2401 Taft Avenue, Manila 0922, Philippines.

4Chemistry Department, De La Salle University, 2401 Taft Avenue, Manila 0922, Philippines.

5National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1, Li-Nong St., Sec. 2, Taipei 112, Taiwan.

6Chemistry Department, De La Salle University Science & Technology Complex, Leandro V. Locsin Campus, Biñan City, Laguna 4024, Philippines.

Open Access   

Published:  Nov 27, 2015

DOI: 10.7324/JAPS.2015.501104
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Abstract

The triterpenes, squalene (1), friedelin (2) and a mixture of ursolic acid (3a) and oleanolic acid (3b) in a 2:3 ratio, and a mixture of β-sitosterol (4a) and stigmasterol (4b) in a 2:1 ratio, obtained from the dichloromethane extract of Pipturus arborescens (Link) C.B. Rob., were evaluated for their anti-proliferative activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast- neonatal (HDFn) using the in vitro PrestoBlue® cell viability assay. The HCT-116 cell line was most susceptible to the compounds and mixtures tested. Triterpene 1 was most cytotoxic against HCT-116 and MCF-7 with IC50 values of 4.21 and 5.92 μg/mL, respectively. Triterpene 2 and the mixture of 3a and 3b were highly anti-proliferative against HCT-116 cells (IC50 of 1.22 and 1.66 μg/mL, respectively) and moderately inhibitory against MCF-7 cells (IC50 of 16.51 and 23.97 μg/mL, respectively). The mixture of 4a and 4b exhibited high cytotoxicity against HCT-116 cells (IC50 of 1.14 μg/mL). Compounds 1-4b showed the least activity against HT-29 cells (IC50 of 11.97 to 52.52 μg/mL). Cytotoxic effect was not observed against HDFn cells (>100 μg/mL). Comparing the effects of 1-4b on the two colon cancer cell lines, the IC50 values of 1-4b against HCT-116 were lower than those of HT-29.


Keyword:     Pipturus arborescens (Link) C.B. Rob. squalene friedelin ursolic acid oleanolic acid β-sitosterol stigmasterol cytotoxicity MCF-7 HCT-116 HT-29 HDFn.

Citation:

Mariquit M. De Los Reyes, Glenn G. Oyong, Virgilio D. Ebajo Jr., Vincent Antonio S. Ng, Chien-Chang Shen, Consolacion Y. Ragasa. Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob. J App Pharm Sci, 2015; 5 (11): 023- 030.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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