Antibacterial activity di-2-ethylaniline phosphate was studied against four Gram negative bacteria at 100-10000 µg/ml concentration using the paper disc diffusion method. Results indicated that the test compound, di-2-ethylaniline phosphate has demonstrated significant antibacterial activity against all selected Gram negative bacteria. Di-2-ethylaniline phosphate was found to be effective against bacteria A at lowest concentration 100 µg/ml where the zone of inhibition was 9 mm diameter in size and largest zone 24 mm diameter formed at highest concentration 10000 µg/ml against bacteria D while bacteria B and C exhibited more resistance as compared to bacteria A and D. In conclusion di-2-ethylaniline phosphate exhibited an efficient antibacterial activity.
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