Biological activity, chemical profiling and molecular docking of tissue extracts of the sea snail Trochus erithreus

Khaled M. Zayed Mosad A. Ghareeb Mohamed R. Habib Hanaa M. Abu El-Einin Rasha E.M. Ali Rasha M. Gad El-Karim Rehab Sabour Ahmed A. Hamed   

Open Access   

Published:  Jan 10, 2023

DOI: 10.7324/JAPS.2023.119765

The biodiversity of marine mollusks, along with their worldwide availability and ease of harvesting, makes them an enticing subject for scientific research. Mollusks are frequently the target of pharmaceutical scientists who are searching for novel compounds to benefit the population. This study targets the investigation of the antioxidant, antibiofilm, antimicrobial, and cytotoxic activities of different solvent extracts of marine mollusk Trochus erithreus. The chemical constituents of the most promising extract and molecular docking analyses are also investigated. Results showed that ethyl alcohol extract had the highest phenolic content (213.90 ± 4.88 mg GAE/ g extract), total antioxidant activity (545.33 ± 4.02 mg AAE/ g extract), and antibacterial activity against Staphylococcus aureus (31.32 ± 1.49), while the acetone extract had the highest scavenging effect against 2,2-diphenyl-1-picryl-hydrazyl radical (IC50: 53.59 ± 1.71 μg/ml). The acetone extract also displayed pronounced antibacterial activity against Proteus vulgaris with an inhibition ratio of up to 67.93% and it had potent cytotoxic effects against both prostate (IC50: 1.666 ± 0.07 μg/ml) and human lung carcinoma cell lines (IC50: 12.04 ± 0.55 μg/ml). Gas chromatography-mass spectrometry investigation of the acetone extract resulted in the recognition of 45 compounds. Bisabolol oxide A (24.63%), (E)-α-farnesene (7.89%), and tricyclo[,5)] dodeca-3,6,8,11-tetraene (5.41%) were detected as major components. The molecular docking study demonstrated strong binding scores and modes of interactions of several major compounds, inside the active binding sites of bacterial enzymes, thymidylate kinase, DNA gyrase B, and DNA topoisomerase IV/subunit Bs.

Keyword:     Marine mollusca chemical constituents molecular docking bioactivities antimicrobial antibiofilm


Zayed KM, Ghareeb MA, Habib MR, El-Einin HMA, Ali REM, El-Karim RMG, Sabour R, Hamed AA. Biological activity, chemical profiling and molecular docking of tissue extracts of the sea snail Trochus erithreus. J Appl Pharm Sci, 2023.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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