Liquid Chromatography and Fourier Transform Infrared Spectroscopy for quantitative analysis of individual and total curcuminoid in Curcuma longa extract

Ratna Wulandari; Sudjadi; Sudibyo Martono; Abdul Rohman

doi:10.7324/JAPS.2018.8916

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Abstract

Analysis of individual and total curcuminoid composed of curcumin (CUR), desmethoxycurcumin (DMCUR) and bisdemethoxycurcumin (BDMCUR) in Curcuma species is very important. Curcuminoids are frequently used as markers in herbal medicine involving the use of Curcuma species in its formulation. The objective of this study was to validate high-performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy-partial least square (FTIR-PLS) for quantitative analysis of total and individual curcuminoids in turmeric (Curcuma longa L.) extract. The turmeric powder was macerated using ethanol and evaporated to obtain an ethanolic extract. The actual contents of individual curcuminoids of CUR, DMCUR, and BDMCUR were determined using HPLC. FTIR-PLS can be used for quantitative analysis of individual and total curcuminoids using specific wavenumbers. The coefficient of determination (R2) for the relationship between actual values and FTIR-PLS predicted values for calibration, internal, and external validation was >0.98 which indicated good accuracy. The relatively low values of errors in calibration, validation and external validation indicated good precision. FTIR spectroscopy-PLS can be used as an alternative technique over HPLC for determination of individual and total curcuminoid in turmeric extracts.

Keyword: Curcuma longa Linn curcuminoid partial least square HPLC FTIR spectroscopy.

Citation:

Wulandari R, Sudjadi, Martono S, Rohman A. Liquid Chromatography and Fourier Transform Infrared Spectroscopy for quantitative analysis of individual and total curcuminoid in Curcuma longa extract. J App Pharm Sci, 2018; 8(09): 107-113.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reference

Aggarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: Preclinical and clinical studies. Anticancer Res, 2003; 23:363- 398.
Anubala S, Sekar R, Nagaiah K. Development and validation of an analytical method for the separation and determination of major bioactive curcuminoids in Curcuma longa rhizomes and herbal products using non-aqueous capillary electrophoresis. Talanta, 2014; 123:10-17.https://doi.org/10.1016/j.talanta.2014.01.017
Asai A, Miyazawa T. Occurrence of orally administered curcuminoid as glucuronide and glucuronide/sulfate conjugates in rat plasma. Life Sci, 2000; 67:2785-2793.https://doi.org/10.1016/S0024-3205(00)00868-7
Bunaciu AA, Aboul-Enein HY, Fleschin S. Recent Applications of Fourier Transform Infrared Spectrophotometry in Herbal Medicine Analysis. Appl Spectros Rev, 2011; 46:251-260.https://doi.org/10.1080/05704928.2011.565532
Cheng J, Weijun K, Yun L, Jiabo W, Haitao W, Qingmiao L, Xiaohe X. Development and validation of UPLC method for quality control of Curcuma longa Linn.: Fast simultaneous quantitation of three curcuminoids. J Pharm Biomed Anal, 2010; 53:43-49.https://doi.org/10.1016/j.jpba.2010.03.021
De R, Kundu P, Swarnakar S, Ramamurthy T, Chowdhury A, Nair GB, Mukhopadhyay AK. Antimicrobial Activity of Curcumin against Helicobacter pylori Isolates from India and during Infections in Mice. Antimicrob Agents Chemother, 2009; 53:1592-1597.https://doi.org/10.1128/AAC.01242-08
Gad HA, El-Ahmady SH, Abou-Shoer MI, Al-Azizi MM. Application of chemometrics in authentication of herbal medicines: A review. Phytochem Anal, 2013; 24:1-24.https://doi.org/10.1002/pca.2378
González AG, Herrador MÁ. A practical guide to analytical method validation, including measurement uncertainty and accuracy profiles. TrAC Trends Anal Chem, 2007; 26:227-238.https://doi.org/10.1016/j.trac.2007.01.009
Gupta AP, Gupta MM, Kumar S. Simultaneous Determination of Curcuminoids in Curcuma Samples Using High Performance Thin Layer Chromatography. J Liq Chromatogr Related Technol, 1999; 22:1561-1569.https://doi.org/10.1081/JLC-100101751
Inoue K, Nomura C, Ito S, Nagatsu A, Hino T, Oka H. Purification of curcumin, demethoxycurcumin, and bisdemethoxycurcumin by high-speed countercurrent chromatography. J Agric Food Chem, 2008; 56:9328-9336.https://doi.org/10.1021/jf801815n
International Conference on Harmonisation. Validation of Analytical Procedures: Text and Methodology. 2005; Retrieved from http:// www.ich.org/products/guidelines.html.
Issuriya A, Kumarnsit E, Wattanapiromsakul C, Vongvatcharanond U. Histological studies of neuroprotective effects of Curcuma longa Linn. on neuronal loss induced by dexamethasone treatment in the rat hippocampus. Acta Histochemica, 2014; 116:1443-1453.https://doi.org/10.1016/j.acthis.2014.09.009
Jiang H, Somogyi A, Jacobsen NE, Timmermann BN, Gang DR. Analysis of curcuminoids by positive and negative electrospray ionization and tandem mass spectrometry. Rapid Comm Mass Spectrom, 2006; 20:1001-1012.https://doi.org/10.1002/rcm.2401
Lestari HP, Martono S, Sudjadi, Rohman A. Simultaneous analysis of Curcumin and demethoxycurcumin in Curcuma xanthorriza using FTIR spectroscopy and chemometrics. Int Food Res J, 2017; 24(5):2097-2101.
Liang YZ, Xie P, Chan K. Quality control of herbal medicines. J Chromatogr B, 2004; 812:53-70.https://doi.org/10.1016/S1570-0232(04)00676-2
Miller JN, Miller JC. 2005. Statistics and Chemometrics for Analytical Chemistry. 5th Ed. Edinburgh: Pearson Education Limited.
Dandekar PP, Patravale VB. Development and Validation of a Stability-Indicating LC Method for Curcumin. Chromatographia, 2009; 69:871-877.https://doi.org/10.1365/s10337-009-0995-1
Paulucci VP, Couto RE, Teixeira CCC, Freitas LAP. Optimization of the extraction of curcumin from Curcuma longa rhizomes. Brazilian J Pharmacog, 2013; 23:94-100.https://doi.org/10.1590/S0102-695X2012005000117
Péret-Almeida L, Cherubino APF, Alves RJ, Dufossé L, Glória MBA. Separation and determination of the physico-chemical characteristics of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Food Res Int, 2005; 38:1039-1044.https://doi.org/10.1016/j.foodres.2005.02.021
Prabaningdyah NK, Riyanto S, Rohman A, Siregar C. Application of HPLC and response surface methodology for simultaneous determination of curcumin and desmethoxy curcumin in Curcuma syrup formulation. J App Pharm Sci, 2017; 7:58-64.
Prabaningdyah NK, Riyanto S, Rohman A. Application of FTIR spectroscopy and multivariate calibration for analysis of curcuminoid in syrup formulation. J App Pharm Sci, 2018; 8:172-179.
Rogers NM, Kireta S, Coates PTH. Curcumin induces maturation-arrested dendritic cells that expand regulatory T cells in vitro and in vivo. Clin Exp Immun, 2010; 162:460-473.https://doi.org/10.1111/j.1365-2249.2010.04232.x
Rohaeti E, Rafi M, Syafitri UD, Heryanto R. Fourier transform infrared spectroscopy combined with chemometrics for discrimination of Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar. Spectrochim. Acta Part A: Mol Biomol Spectros, 2015; 137:1244-1249.https://doi.org/10.1016/j.saa.2014.08.139
Rohman A. Application of Fourier Transform Infrared Spectroscopy for Quality Control of Pharmaceutical Products: A Review. Indonesian J Pharm, 2012; 23:1-8.
Rohman A, Sudjadi D, Ramadhani D, Nugroho A. Analysis of Curcumin in Curcuma longa and Curcuma xanthorriza Using FTIR Spectroscopy and Chemometrics. Res J Med Plant, 2015; 9:179-186. https://doi.org/10.3923/rjmp.2015.179.186
Singh R, Chandra R, Bose M, Luthra PM. Antibacterial activity of Curcuma longa rhizome extract on pathogenic bacteria. Current Sci, 2002; 83:737-740.
Syed HK, Liew KB, Loh GOK, Peh KK. Stability indicating HPLC–UV method for detection of curcumin in Curcuma longa extract and emulsion formulation. Food Chem, 2015; 170:321-326. https://doi.org/10.1016/j.foodchem.2014.08.066
Tanaka K, Kuba Y, Sasaki T, Hiwatashi F, Komatsu K. Quantitation of curcuminoids in curcuma rhizome by near-infrared spectroscopic analysis. J Agric Food Chem, 2008; 56:8787-8792. https://doi.org/10.1021/jf801338e
The Ministry of Health, Republic of Indonesia. 2008. Indonesian Herbal Pharmacopeia. 1st Ed. Jakarta: Kemenkes.
Wichitnithad W, Jongaroonngamsang N, Pummangura S, Rojsitthisak P. A simple isocratic HPLC method for the simultaneous determination of curcuminoids in commercial turmeric extracts. Phytochem Anal, 2009; 20:314-319. https://doi.org/10.1002/pca.1129
Wickenberg J, Ingemansson SL, Hlebowicz J. Effects of Curcuma longa (turmeric) on postprandial plasma glucose and insulin in healthy subjects. Nutr J, 2010; 9:1-5. https://doi.org/10.1186/1475-2891-9-43
Wilken R, Veena MS, Wang MB, Srivatsan ES. Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma. Mol Cancer, 2011; 10:1-19. https://doi.org/10.1186/1476-4598-10-12

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