Effect of Kirganelia reticulata extract on Human Embryonic Kidney cells with evidence on morphological changes

Madhurima Ghosh; Jhinuk Chatterjee; V. Krishnamurthy

doi:10.7324/JAPS.2018.8917

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Abstract

Kirganelia reticulata has been proven to have cytotoxic activities. The aim of this study is to demonstrate the anti-proliferative activity of the methanolic extract of K. reticulata on Human Embryonic Kidney cells (HEK-293). Changes in cellular morphology of HEK-293 cells were evident at higher concentration and with an increase in time, which was supported by our experimental data. Our preliminary data suggest that K. reticulata methanolic extract has anti-proliferative activity.

Keyword: Phytochemicals Kirganelia HEK-293 Cell morphology Anti-Proliferative.

Citation:

Ghosh M, Chatterjee J, Krishnamurthy V. Effect of Kirganelia reticulata extract on Human Embryonic Kidney cells with evidence on morphological changes. J App Pharm Sci, 2018; 8(09): 114-118.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sandercock AM, Rust S, Guillard S, Sachsenmeier KF, Holoweckyj N, Hay C, Flynn M, Huang Q, Yan K, Herpers B, Price LS, Soden J, Freeth J, Jermutus L, Hollingsworth R, Minter R. Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling. Molecular Cancer, 2015; 14:147. https://doi.org/10.1186/s12943-015-0415-0
Joshi B, Barman I, Dingari NC, Cardenas N, Soares JS, Dasari RR, Samarendra Mohanty. Label-free route to rapid, nanoscale characterization of cellular structure and dynamics through opaque media. Scientific Reports, 2013; 3:2822. https://doi.org/10.1038/srep02822
Haass-Koffler CL, Naeemuddin M, Bartlett SE. An analytical tool that quantifies cellular morphology changes from three dimensional fluorescence images. Journal of Visualized Experiments, 2012; 66:e4233. Chopra RN, Nayar SL, Chopra IC. 1956. Glossary of Indian medicinal plants (2nd Edition), New Delhi, India: CSIR. Ghani A. 2003. Medicinal plants of Bangladesh, chemical constituents and uses (2nd Edition), Asiatic Society of Bangladesh 345.
Kataria H, Shah N, Kaul SC, Wadhwa R, Kaur G. Water extract of ashwagandha leaves limits proliferation and migration, and induces differentiation in glioma cells. Evidence-Based Complementary and Alternative Medicine, 2011; 2011, ID 267614.
Joshi KC, Singh P, Mehta A. Crystalline components of the root of Phyllanthus reticulata. Journal of Indian Chemical Society, 1991; 58:102.
Kumar S, Kumar D, Deshmukh RR, Lokhande PD, More SN, Rangari VD. Antidiabetic potential of Phyllanthus reticulata in alloxan-induced diabetic mice. Fitoterapia, 2008; 79:21-23. https://doi.org/10.1016/j.fitote.2007.06.012
Mehta RG, Pezzuto JM. Discovery of cancer preventive agents from natural products: From plants to prevention. Current Oncology Reports, 2002; 4:478-486. https://doi.org/10.1007/s11912-002-0059-2
Michaud DS, Feskanich D, Rimm EB. Intake of specific carotenoids and risk of lung cancer in two prospective U.S. cohorts. American Journal of Clinical Nutrition, 2000; 72(4):990-997. https://doi.org/10.1093/ajcn/72.4.990
Merlin NJ, Parthasarathy V, Santhoshkumar TR. Induction of apoptosis in human breast cancer cell line MCF-7 by phytochemicals from Gmelina asiatica. African Journal of Biotechnology, 2010; 9(28):4451- 4456.
Lampronti I, Martello D, Bianchi N, Borgatti M, Lambertini E, Piva R, Jabbar S, Choudhuri MSK, Khan MTH, Gambari R. In vitro antiproliferative effects on human tumor cell lines of extracts from the Bangladeshi medicinal plant Aegle marmelos Correa. Phytomedicine, 2003; 10(4):300-308. https://doi.org/10.1078/094471103322004794
Reddy RA, Madgaonkar V, Venkatesh RT, Bharathi R, Krishna Murthy V. Potential cytotoxic drug effects of secondary metabolites derived from selected medicinal plants of Savanadurga forest in Karnataka. International Journal of Pharmacy and Pharmaceuticals Sciences, 2014; 6(10):238-241.
Guan R, Kang T, Lu F, Zhang Z, Shen H, Liu M. Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles. Nanoscale Research Letters, 2012; 7(1):602. https://doi.org/10.1186/1556-276X-7-602
Russo M, Spagnuolo C, Tedesco I, Russo GL. Phytochemicals in Cancer Prevention and Therapy. Toxins, 2010; 2:517-551. https://doi.org/10.3390/toxins2040517
Suresh Kumar MA, Mangalam N, Hema PS, John M, Santhosh Kumar TR. Pinocembrin triggers Bax-dependent mitochondrial apoptosis in colon cancer cells. Mol Carcinogen, 2007; 46(3):231-241. https://doi.org/10.1002/mc.20272
Haghparast SMA, Kihara T, Miyake J. Distinct mechanical behavior of HEK293 cells in adherent and suspended states. Peer J, 2015; 3:e1131. https://doi.org/10.7717/peerj.1131
Shruthi SD, Ramachandra YL, Padmalatha Rai S, Jha PK. Pharmacognostic of the leaves of Kirganelia reticulata Baill (Euphorbiaceae). The Asian and Australasian Journal of Plant Sciences and Biotechnology, 2010; 4(1):62-65.
Unander DW, Webster GL, Blumberg BS. Records of usage or assays in Phyllanthus (Euphorbiaceae) L. Subgenera Isocladus, Kirganelia, Cicca, Emblica. Journal of Ethnopharmacol, 1990; 30(3):233-64. https://doi.org/10.1016/0378-8741(90)90105-3
Selvaraj V, Bodapati S, Murray E, Rice KM, Winston N, Shokuhfar T, Zhao Y, Blough E. Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells. International Journal of Nanomedicine, 2014; 9:1379-1391. https://doi.org/10.2147/IJN.S52625

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Sandercock AM, Rust S, Guillard S, Sachsenmeier KF, Holoweckyj N, Hay C, Flynn M, Huang Q, Yan K, Herpers B, Price LS, Soden J, Freeth J, Jermutus L, Hollingsworth R, Minter R. Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling. Molecular Cancer, 2015; 14:147. https://doi.org/10.1186/s12943-015-0415-0

Joshi B, Barman I, Dingari NC, Cardenas N, Soares JS, Dasari RR, Samarendra Mohanty. Label-free route to rapid, nanoscale characterization of cellular structure and dynamics through opaque media. Scientific Reports, 2013; 3:2822. https://doi.org/10.1038/srep02822

Haass-Koffler CL, Naeemuddin M, Bartlett SE. An analytical tool that quantifies cellular morphology changes from three dimensional fluorescence images. Journal of Visualized Experiments, 2012; 66:e4233. Chopra RN, Nayar SL, Chopra IC. 1956. Glossary of Indian medicinal plants (2nd Edition), New Delhi, India: CSIR. Ghani A. 2003. Medicinal plants of Bangladesh, chemical constituents and uses (2nd Edition), Asiatic Society of Bangladesh 345.

Kataria H, Shah N, Kaul SC, Wadhwa R, Kaur G. Water extract of ashwagandha leaves limits proliferation and migration, and induces differentiation in glioma cells. Evidence-Based Complementary and Alternative Medicine, 2011; 2011, ID 267614.

Joshi KC, Singh P, Mehta A. Crystalline components of the root of Phyllanthus reticulata. Journal of Indian Chemical Society, 1991; 58:102.

Kumar S, Kumar D, Deshmukh RR, Lokhande PD, More SN, Rangari VD. Antidiabetic potential of Phyllanthus reticulata in alloxan-induced diabetic mice. Fitoterapia, 2008; 79:21-23. https://doi.org/10.1016/j.fitote.2007.06.012

Mehta RG, Pezzuto JM. Discovery of cancer preventive agents from natural products: From plants to prevention. Current Oncology Reports, 2002; 4:478-486. https://doi.org/10.1007/s11912-002-0059-2

Michaud DS, Feskanich D, Rimm EB. Intake of specific carotenoids and risk of lung cancer in two prospective U.S. cohorts. American Journal of Clinical Nutrition, 2000; 72(4):990-997. https://doi.org/10.1093/ajcn/72.4.990

Merlin NJ, Parthasarathy V, Santhoshkumar TR. Induction of apoptosis in human breast cancer cell line MCF-7 by phytochemicals from Gmelina asiatica. African Journal of Biotechnology, 2010; 9(28):4451- 4456.

Lampronti I, Martello D, Bianchi N, Borgatti M, Lambertini E, Piva R, Jabbar S, Choudhuri MSK, Khan MTH, Gambari R. In vitro antiproliferative effects on human tumor cell lines of extracts from the Bangladeshi medicinal plant Aegle marmelos Correa. Phytomedicine, 2003; 10(4):300-308. https://doi.org/10.1078/094471103322004794

Reddy RA, Madgaonkar V, Venkatesh RT, Bharathi R, Krishna Murthy V. Potential cytotoxic drug effects of secondary metabolites derived from selected medicinal plants of Savanadurga forest in Karnataka. International Journal of Pharmacy and Pharmaceuticals Sciences, 2014; 6(10):238-241.

Guan R, Kang T, Lu F, Zhang Z, Shen H, Liu M. Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles. Nanoscale Research Letters, 2012; 7(1):602. https://doi.org/10.1186/1556-276X-7-602

Russo M, Spagnuolo C, Tedesco I, Russo GL. Phytochemicals in Cancer Prevention and Therapy. Toxins, 2010; 2:517-551. https://doi.org/10.3390/toxins2040517

Suresh Kumar MA, Mangalam N, Hema PS, John M, Santhosh Kumar TR. Pinocembrin triggers Bax-dependent mitochondrial apoptosis in colon cancer cells. Mol Carcinogen, 2007; 46(3):231-241. https://doi.org/10.1002/mc.20272

Haghparast SMA, Kihara T, Miyake J. Distinct mechanical behavior of HEK293 cells in adherent and suspended states. Peer J, 2015; 3:e1131. https://doi.org/10.7717/peerj.1131

Shruthi SD, Ramachandra YL, Padmalatha Rai S, Jha PK. Pharmacognostic of the leaves of Kirganelia reticulata Baill (Euphorbiaceae). The Asian and Australasian Journal of Plant Sciences and Biotechnology, 2010; 4(1):62-65.

Unander DW, Webster GL, Blumberg BS. Records of usage or assays in Phyllanthus (Euphorbiaceae) L. Subgenera Isocladus, Kirganelia, Cicca, Emblica. Journal of Ethnopharmacol, 1990; 30(3):233-64. https://doi.org/10.1016/0378-8741(90)90105-3

Selvaraj V, Bodapati S, Murray E, Rice KM, Winston N, Shokuhfar T, Zhao Y, Blough E. Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells. International Journal of Nanomedicine, 2014; 9:1379-1391. https://doi.org/10.2147/IJN.S52625