Published:  Dec 30, 2017DOI: 10.7324/JAPS.2017.71207
The effectiveness of herbal medicine containing Curcuma extract depends on the active compounds of curcumin (CUK) and desmethoxycurcumin (DMCUR) present in formulation. The objective of this study was to optimize HPLC condition using experimental design of Box-Behnken design (BBD) for simultaneous determination of C and DMC in syrup containing curcuma extract. The column used was RP 18 Waters® X-Bridge (250 x 4.6 mm i.d.; 5 µm). Four variables namely column temperature, flow rate, solvent ratio of acetonitrile-acetic acid, and % acetic acid were used and subjected to RSM in order to get optimum condition. The predicted optimum assay condition consisted of mobile phase acetonitrile-acetic acid 4.08% (49: 51) delivered isocratically at flow rate 1.04 mL/min with column temperature of 40oC. Using this optimum condition, baseline separation of both compounds was achieved with good resolution. The optimized HPLC assay condition was validated according to International conference on Harmonization (ICH) guidelines by assessing several parameters such as speciï¬city, linearity, accuracy, sensitivity and precision.
Prabaningdyah NK, Riyanto S, Rohman A, Siregar C. Application of HPLC and response surface methodology for simultaneous determination of curcumin and desmethoxycurcumin in Curcuma syrup formulation. J App Pharm Sci, 2017; 7 (12): 058-064.
409 Absract views 19 PDF Downloads 428 Total views
Liquid Chromatography and Fourier Transform Infrared Spectroscopy for quantitative analysis of individual and total curcuminoid in Curcuma longa extract
Soluble Curcumin: A Promising Oral Supplement For Health ManagementPuneet Gandhi, Zeba Khan, Nivedita Chakraverty