Research Article | Volume: 7, Issue: 7, July, 2017

In silico Assessment of the Genotypic Distribution of Virulence and Antibiotic Resistance Genes in Pseudomonas aeruginosa

Nusrat Nahar Sharmeen Asad Tufael Ahmed Nurul Islam Setu Md. Shahidulla Kayser Md. Shariful Islam Md. Kamrul Islam Muhammed Mahfuzur Rahman D. A. Anwar Al Aman Ridwan Bin Rashid   

Open Access   

Published:  Jul 30, 2017

DOI: 10.7324/JAPS.2017.70710
Abstract

Pseudomonas aeruginosa harbours virulence and antibiotic resistance genes that contribute to life-threatening infections. In silico PCR amplification detected both virulence and antibiotic resistance genes of eighteen Pseudomonas aeruginosa isolates. L lipoprotein (oprL) gene was found in 72.22% of the isolates and is used usually to rapidly identify Pseudomonas aeruginosa species. Alkaline protease (apr) was detected in 66.67% of the isolates. Protein biosynthesis inhibited by exotoxin A (exoA), was found in 55.56% of the isolates. Fifty percent (n=9) of the isolates had the exoenzyme S (exoS) and elastase (lasB) genes. Thirteen isolates (72.22%) which harboured the phospholipid C (plcH) genes, were also found to be positive for rhamnolipid AB (rhlAB) genes. Thirteen isolates (72.22%) were found to possess alginate (algD) and neuraminidae (nan2) genes, respectively. Pseudomonas aeruginosa PA7 harboured both aph(3”) and aph(6)-1d genes. Only sulfonamide resistance gene, sul1 was present in 11.11% (n=2) of the isolates. Beta-lactamase gene, blaTEM was present in only one isolate and no tetracycline resistance gene was found. Fluoroquinolone resistance-determining region of the gyrA and parC genes were present in fourteen (77.78%) and thirteen (72.22%) isolates, respectively. Pseudomonas aeruginosa NCGM2.S1 is a multidrug resistant bacterium since it harboured class 1 integrase gene. In silico pulsed-field gel electrophoresis (PFGE) analysis was able to group eighteen isolates into three genotypes. Gene distribution pattern within genotypes was almost similar and not dependent on genotypes. The data generated here helps to predict virulence and antibiotic resistance profile of Pseudomonas aeruginosaspecies based on genotype.


Keyword:     Antibiotic resistance genes Genotype PCR Pseudomonas aeruginosa Pulsed-field gel electrophoresis Virulence genes.


Citation:

Nahar N, Asad S, Ahmed T, Setu NI, Kayser MS, Islam MS, Islam MK, Rahman MM, Al Aman DAA, Rashid RB. In silico assessment of the genotypic distribution of virulence and antibiotic resistance genes in Pseudomonas aeruginosa. J App Pharm Sci, 2017; 7 (07): 055-061.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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