Research Article | Volume: 7, Issue: 7, July, 2017

Simultaneous quantification of ramipril, glimepiride and metformin in human plasma by ultra-performance liquid chromatography – tandem mass spectrometry

Eman S. Elzanfaly Sherif A. Abdel-Gawad   

Open Access   

Published:  Jul 30, 2017

DOI: 10.7324/JAPS.2017.70711

Ramipril (RAM), glimepiride (GLM) and metformin (MET) are recently formulated together for the treatment of diabetes and cardiovascular disorders. This work introduces a liquid chromatography–tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of the three drugs in human plasma after liquid-liquid extraction. This method made use of atorvastatin as internal standard (IS). Analytes were recovered from plasma by n-hexane: butanol (50:50%, v/v) and subsequently separated on Waters AcquityTM UPLC BEH shield RP-C18 column using methanol: water containing 0.1% formic acid (90: 10%, v/v) as a developing system. The calibration curves were linear (r2 > 0.99) over a range of 0.1 - 1000 ng/mL for RAM & GLM and 250 - 2000 ng/mL for MET. The intra-day and inter-day precisions were below 14.32% and the accuracy was all within ±15%. Moreover, other validation parameters for the proposed method like matrix effect, selectivity, recovery and stability were adopted. The proposed method can be applied for the sensitive and selective quantification of the analytes in bioavailability and pharmacokinetics studies.

Keyword:     Human plasma liquid chromatography ramipril glimepiride metformin.


Elzanfaly ES, Abdel-Gawad SA. Simultaneous quantification of ramipril, glimepiride and metformin in human plasma by ultraperformance liquid chromatography – tandem mass spectrometry. J App Pharm Sci, 2017; 7 (07): 062-069.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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