Research Article | Volume: 7, Issue: 6, June, 2017

Total phenolics and flavonoids content, antioxidant activity and GC/MS analyses of Euphorbia grandialata

Mona Ismaila Asmaa I. Owisa Mona Hettab Rabab Mohammeda   

Open Access   

Published:  Jun 30, 2017

DOI: 10.7324/JAPS.2017.70625

Objective: This study has been designed to study phenolic and flavonoid contents quantitatively; screen antioxidant activity and investigate unsaponifiable and saponifiable matters of Euphorbia grandialata R. aerial parts.

Methods: The phenolics and flavonoids content were assayed by Folin-Ciocalteu reagent and aluminium chloride reagent respectively, while the anti-oxidant activity was done by 2, 2-diphenyl-1-picrylhydrazyl (DPPH).GC/MS analysis was used to analyze unsaponifiable and saponifiable matters.

Results: Total phenolics result was calculated as (17.61 ± 1.2 μg gallic acid/g), total flavonoids as (4.495 ± 0.39 μg rutin/g) and anti-oxidants activity as (140.6 % μg ascorbic acid/g). The identified compounds in unsaponifiable matter were hydrocarbons (51.1 %), steroids and triterpenes (35.92%). The saponifiable matter showed fourteen components from which six were identified as methyl esters of saturated fatty acids (46.26%) and eight as methyl esters of unsaturated fatty acids (53.74 %).

Conclusion: Euphorbia grandialata could be considered as a valuable source of natural antioxidants. This is the first report for phenolics and flavonoids content, antioxidant activity and GC/MS analysis of this plant.

Keyword:     Euphorbiaceae phenolics DPPH GC/MS.


Ismail M, Owis AI, Hetta M, Mohammed R. Total phenolics and flavonoids content, antioxidant activity and GC/MS analyses of Euphorbia grandialata. J App Pharm Sci, 2017; 7 (06): 176-181.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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