Research Article | Volume: 5, Issue: 2, February, 2015

Cytotoxic compounds from the leaves of Garcinia cowa Roxb.

Fatma Sri Wahyuni Khozirah Shaari Johnson Stanslas Nordin Lajis Dachriyanus Hamidi   

Open Access   

Published:  Feb 27, 2015

DOI: 10.7324/JAPS.2015.50202
Abstract

The aims of this study was to isolate compounds from the leaves of methanol extract of Garcinia cowa and to evaluated their cytotoxic activity against breast (MCF-7) and lung (H-460) cell lines. The dichloromethane fraction was separated by successive silica gel column chromatography to give three compounds. Based on spectroscopic comparison with those of the literature these compounds were elucidated as methyl 2,4,6-trihydroxy-3-(3-methylbut-2-enyl)benzoate (1), garcinisidone-A (2) and methyl 4,6dihydroxy-2-(4-methoxy-5-(3-methylbut-2-enyl)-3,6-dioxocylohexa-1,4-dienyloxy)-3-(3-methylbut-2-enyl)benzoate (3). Compound 1, 2 and 3 had IC50 value of 21.0 ± 10.2 µM, 21.2 ±8.4 µM and 17.2 ± 6.2µM against MCF-7, while only compound (2) was found to be in active against H-460 with IC50 value of 18.1 ± 6.7 µM. Conclusion: The results indicate that G. cowa leaves could be important sources of natural cytotoxic compounds and only compound (2) had activity against H-460 cell lines.


Keyword:     Structure elucidation NMR spectroscopy xanthone benzoate Garcinia cowa Roxb. cytotoxic activity MCF-7 H-460.


Citation:

Fatma Sri Wahyuni, Khozirah Shaari, Johnson Stanslas, Nordin Hj Lajis, Dachriyanus Hamidi., Cytotoxic compounds trom the leaves of Garcinia Cowa Roxb. J App Pharm Sci, 2015; 5 (02): 006-011.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
495 Views 84 Downloads 579 Total

Year

Month

Related Search

By author names