Research Article | Volume: 3, Issue: 1, January, 2013

Analytical method development and validation of sitagliptine phosphate monohydrate in pure and tablet dosage form by derivative spectroscopy

G. Jeyabalan and Narendra Nyola   

Open Access   

Published:  Jan 28, 2013

DOI: 10.7324/JAPS.2013.30118
Abstract

In this study zero, first and second order derivative spectrophotometric method were developed for the estimation of sitagliptine. In zero order spectrophotometery, absorbance value was measured at 267nm. In first derivative spectrophotometry amplitudes were measured at 213nm. In second derivative spectrophotometry amplitudes were measured at 276nm. Calibration curves were linear between the concentration range of 20-60µg/ml, 20-60µg/ml and 40-80µg/ml respectively. The % RSD value is less than 2% and the recovery were near 100% for all methods. This method has been validated for linearity, accuracy and precision and found to be rapid, precise, accurate and economical and can be applied for routine estimation of sitagliptine in solid dosage form. The validation of method was carried out utilizing ICH-guidelines.


Keyword:     Spectrophotometric method Sitagliptine Derivative spectroscopy.


Citation:

G. Jeyabalan and Narendra Nyola. Analytical method development and validation of sitagliptine phosphate monohydrate in pure and tablet dosage form by derivative spectroscopy. J App Pharm Sci. 2013; 3 (01): 095-098.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics

599 Absract views 6 PDF Downloads 605 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required