Development and validation of spectrophotometric method for simultaneous estimation of metoprolol succinate and olmesartan medoxomil in tablet

AbstractThe present manuscript describes simple, sensitive, rapid, accurate, precise and economic dual wavelength spectrophotometric method for simultaneous determination of metoprolol succinate and olmesartan medoxomil in combined tablet dosage form. The utility of dual wavelength data processing program is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The wavelengths selected for determination of metoprolol succinate were 225.2 nm and 258.2 nm, whereas the wavelengths selected for determination of olmesartan medoxomil were 211 nm and 229.8 nm. The two drugs follow Beer-Lambert’s law over the concentration range of 5-30 µg/ml. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The results of analysis have been validated statistically and by recovery studies.