Topsin is a fungicide used against a wide range of pathogens in field crops, fruits, ornamentals and vegetables. The present work studied the effect of topsin on the ovary of Wistar rats and the possible ameliorative role of Ginko biloba extract (EGB). Many histopathological changes were seen in the ovary of rats after treatment with topsin. The number of ovarian follicles decreased and most of them degenerated which accompanied by increased of atretic follicles. Topsin significantly decreased the levels of both LH and FSH and increased estradiol. Histochemical results revealed an increase in carbohydrate content and decrease in total protein in the ovarian tissue. Moreover, topsin led to significant increase of LPO and CAT activity and non-significant increase of SOD. Treating animals with topsin and EGB effectively alleviated topsin-induced ovarian toxicity. In conclusion, this study provides evidence that topsin adversely damages ovarian tissue through increasing oxidative stress, while EGB treatment effectively attenuates the toxicity and oxidative effect of topsin in the ovary.