This study aims to evaluate the antibiogram and antibacterial activity of Crassocephalum crepidioides leaf extract against the bacterial strains isolated from infected wounds. A total of 69 swab samples were obtained from various cases of infected wounds and 20 pure bacterial strains were isolated. The most prevalent organisms isolated from wound infections were Staphylococcus species and Escherichia coli (25%), followed by Klebsiella species (15%), Proteus species (10%), Providencia species (10%), Pseudomonas aeruginosa (5%), Acinetobacter baumannii (5%), and Enterobacter hormaechei (5%). The susceptibility pattern of all bacterial isolates was assessed against antibiotic discs using the Kirby Bauer Disc diffusion method. The results revealed that Gram-positive cocci exhibited 100 % susceptibility to Amikacin, Bacitracin, Oxytetracycline, and Vancomycin, however, showed 80% resistance to Novobiocin, Amoxicillin, Cephalothin, Erythromycin. Conversely, Gram-negative bacilli exhibited high resistance levels, including 86.7% to Ciprofloxacin, 80% to Carbericillin and Nitrofurantoin, 66.7% to Streptomycin and Tetracycline, 60% resistance to Co- Trimazine; however, they showed 73.3% sensitivity to Amikacin and 53.3% sensitivity to Kanamycin. Among the 20 bacterial strains, 13 (65%) were identified as multidrug-resistant (MDR) and 4 (20%) were extensively drug-resistant (XDR). In vitro antibacterial activity assay revealed that C. crepidioides leaf extract was found to be effective against all the Staphylococcus spp., E. hormaechei, A. baumannii, Providencia spp., two E. coli isolates and one Klebsiella spp. with the zone size ranging from 10.83 ± 0.28 to 25.83 ± 1.04, with minimum inhibitory concentration between 2.5 and 40 mg/ml, however, resistant to P. aeruginosa, Proteus spp., three E. coli isolates and two Klebsiella spp. Staphylococcus spp. was found to be the most inhibited wound isolates by C. crepidioides leaf extract. These findings suggest that C. crepidioides leaf extract has the potential to develop antibacterial agents against the MDR and XDR organisms causing wound infection, emphasizing the significant role of plant extracts in treating bacterial wound infections, thereby preventing the delay of the wound healing process.
Devi YA, Gnanasekaran P, Devi HJ, Siva D, Seshadri S, Ravindrran MB, Partheeban S, Bhuvaneswari S. Antibiogram and antibacterial activity of Crassocephalum crepidioides (Thickhead) leaf extract against the wound isolates. J Appl Pharm Sci. 2025. Article in Press. http://doi.org/10.7324/JAPS.2025.250483
1. Maillard JY, Kampf G, Cooper R. Antimicrobial stewardship of antiseptics that are pertinent to wounds: the need for a united approach. JAC Antimicrob Resist. 2021;3(1):dlab027. doi: https://doi.org/10.1093/jacamr/dlab027
2. Tasnim A, Alam MS, Yusuf MA, Khan FA, Ferdose J, Sultana M. Prevalence of multidrug resistant (MDR) Proteus spp. in burn wound infection of a Tertiary Care Hospital, Rajshahi. Int J Infect Dis Ther. 2021;6(2):65–8. doi: https://doi.org/10.11648/j.ijidt.20210602.14
3. Tom IM, Ibrahim MM, Umoru AM, Umar JB, Bukar MA, Haruna AB, et al. Infection of wounds by potential bacterial pathogens and their resistogram. Open Access Library J. 2019;6:1–13. doi: https://doi.org/10.4236/oalib.1105528
4. Mama M, Abdissa A, Sewunet T. Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia. Ann Clin Microbiol Antimicrob. 2014;13(1):14. doi: https://doi.org/10.1186/1476-0711-13-14
5. Pallavali RR, Degati VL, Lomada D, Reddy MC, Durbaka, VRP. Isolation and in vitro evaluation of bacteriophages against MDR bacterial isolates from septic wound infections. PLoS ONE. 2017;12(7):179–245. doi: https://doi.org/10.1371/journal.pone.0179245
6. Puca V, Marulli RZ, Grande R, Vitale I, Niro A, Molinaro G, et al. Microbial species isolated from infected wounds and antimicrobial resistance analysis: data emerging from a three-years retrospective study. Antibiotics (Basel). 2021;10(10):1162. doi: https://doi.org/10.3390/antibiotics10101162
7. Taati Moghadam M, Khoshbayan A, Chegini Z, Farahani I, Shariati A. Bacteriophages, a new therapeutic solution for inhibiting multidrug-resistant bacteria causing wound infection: lesson from animal models and clinical trials. Drug Des Dev Ther. 2020;14:1867–83. doi: https://doi.org/10.2147/DDDT.S251171
8. Sandoz H. An overview of the prevention and management of wound infection. Nurs Stand. 2022;37(10):75–82. doi: https://doi.org/10.7748/ns.2022.e11889
9. Williams M. Wound infections: an overview. Br J Community Nurs. 2021;26(6):S22–5. doi: https://doi.org/10.12968/bjcn.2021.26.Sup6.S22
10. Roselinelfeyinwa E. Microbial assessment of wound infection among patients in hospitals within Enugu metropolis. IOSR J Pharm Biol Sci. 2023;18(2):18–23. doi: https://doi.org/10.9790/3008-1802011823
11. Salam MdS, Al-Amin MdY, Salam MT, Pawar JS, Akhter N, Rabaan AA, et al. Antimicrobial resistance: a growing serious threat for global public health. Healthcare (Basel). 2023;11(13):1946. doi: https://doi.org/10.3390/healthcare11131946
12. World Health Organization. WHO global report on traditional and complementary medicine 2019. World Health Organization. Available from: https://iris.who.int/handle/10665/312342
13. Atef NM, Shanab SM, Negm SI, Abbas YA. Evaluation of antimicrobial activity of some plant extracts against antibiotic susceptible and resistant bacterial strains causing wound infection. Bull Natl Res Cent. 2019;43:144. doi: https://doi.org/10.1186/s42269-019-0184-9
14. Mummed B, Abraha A, Feyera T, Nigusse A, Assefa S. In vitro antibacterial activity of selected medicinal plants in the traditional treatment of skin and wound infections in Eastern Ethiopia. Biomed Res Int. 2018;2018:1862401. doi: https://doi.org/10.1155/2018/1862401
15. Abdallah EM, Alhatlani BY, Menezes RdeP, Martins CHGM. Back to nature: medicinal plants as promising drugs in the post-antibiotic era. Plants. 2023;12(17):3077. doi: https://doi.org/10.3390/plants12173077
16. Das A. Evaluation of the effect of Crassocephalum crepidioides on the wound healing in Albino rats. Int J Adv Res. 2022;10:402. doi: http://dx.doi.org/10.21474/IJAR01/15504
17. Devi YA, Gnanasekaran P, Devi HJ. Antibacterial, antioxidant and cytotoxicity assessment of Crassocephalum crepidioides leaf extract. J Pure Appl Microbiol. 2024;18(4):2528–38. doi: https://doi.org/10.22207/JPAM.18.4.24
18. Adedayo BC, Oyeleye SI, Ejakpovi II, and Oboh G. Effects of hot water treatment on the radicals scavenging, lipid peroxidation, and α-amylase and α-glucosidase inhibitory abilities of Crassocephalum crepidioides leaves. Nutrafoods. 2015;14:217–25. doi: https://doi.org/10.1007/s13749-015-0053-6
19. Akinpelu BA, Godwin A, Gbadegesin T, Ajakaye N, Omotosho SE, Azeez SO, et al. Comparative studies on anti-inflammatory, antioxidant and antimutagenic activities of Crassocephalum crepidioides (Bent) leaf cold and hot water extracts. Asian Food Sci J. 2019;9(1):1–12. doi: https://doi.org/10.9734/afsj/2019/v9i130000
20. Abbas A, Naqvi SAR, Rasool MH, Noureen A, Mubarik MS, Tareen RB. Phytochemical analysis, antioxidant and antimicrobial screening of seriphidium oliverianum plant extracts. Dose Response. 2021;19(1):15593258211004739. doi: https://doi.org/10.1177/15593258211004739
21. Ahmed EF, Rasmi AH, Darwish AA, Gad GFM. Prevalence and resistance profile of bacteria isolated from wound infections among a group of patients in upper Egypt: a descriptive cross-sectional study. BMC Res Notes. 2023;16:106. doi: https://doi.org/10.1186/s13104-023-06379-y
22. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 33rd ed. CLSI supplement M100. 2023. Available from: https://clsi.org/about/press-releases/clsi-publishes-m100-performance-standards-for-antimicrobial-susceptibility-testing-33rd-edition/#:~:text=CLSI%20has%20published%20M100%E2%80%94Performance%20Standards%20for%20Antimicrobial%20Susceptibility,standardized%20in%20CLSI%20documents%20M02%2C%20M07%2C%20and%20M11. Accessed 25 October 2024.
23. Owusu E, Ahorlu MM, Afutu E, Akumwena A, Asare GA. Antimicrobial activity of selected medicinal plants from a Sub-Saharan African Country against bacterial pathogens from post-operative wound infections. Med Sci. 2021;9(2):23. doi: https://doi.org/10.3390/medsci9020023
24. Basak S, Singh P, Rajurkar M. Multidrug resistant and extensively drug resistant bacteria: a study. J Pathogens. 2016;2016(1):4065603. doi: https://doi.org/10.1155/2016/4065603
25. Almuhayawi MS, Alruhaili MH, Gattan HS, Alharbi MT, Nagshabandi M, Al Jaouni S, et al. Staphylococcus aureus induced wound infections which antimicrobial resistance, methicillin- and vancomycin-resistant: assessment of emergence and cross-sectional study. Infect Drug Resist. 2023;16:5335–46. doi: https://doi.org/10.2147/IDR.S418681
26. Klink MJ, Laloo N, Taka AL, Pakade VE, Monapathi ME, Modise JS. Synthesis, characterisation and antimicrobial activity of zinc oxide nanoparticles against selected waterborne bacterial and yeast pathogens. Molecules. 2022;27:3532. doi: https://doi.org/10.3390/molecules27113532
27. Gonelimali FD, Lin J, Miao W, Xuan J, Charles F, Chen M, Hatab SR. Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Front Microbiol. 2018;9:1639. doi: https://doi.org/10.3389/fmicb.2018.01639
28. Mostafa AA, Al-Askar AA, Almaary KS, Dawoud TM, Sholkamv EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Bakri Saudi J Biol Sci. 2018;25(2):361–6. doi: https://doi.org/10.1016/j.sjbs.2017.02.004
29. Rai S, Yadav UN, Pant ND, Yakha JK, Tripathi PP, Poudel A, et al. Bacteriological profile and antimicrobial susceptibility patterns of bacteria isolated from pus/wound swab samples from children attending a tertiary care hospital in Kathmandu, Nepal. Int J Microbiol. 2017;2017:2529085. doi: https://doi.org/10.1155/2017/2529085
30. Breijyeh Z, Jubeh B, Karaman R. Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules. 2020;25(6):1340. doi: https://doi.org/10.3390/molecules25061340
31. Miller SI. Antibiotic resistance and regulation of the gram-negative bacterial outer membrane barrier by host innate immune molecules. mBio. 2016;7(5):e01541–16. doi: https://doi.org/10.1128/mBio.01541-16
32. Datta P, Gupta V. Next-generation strategy for treating drug resistant bacteria: antibiotic hybrids. Indian J Med Res. 2019;149:97–106. doi: https://doi.org/10.4103/ijmr.IJMR_755_18
33. Alam MM, Islam MN, Hawlader MDH, Ahmed S, Wahab A, Islam M, et al. Prevalence of multidrug resistance bacterial isolates from infected wound patients in Dhaka, Bangladesh: a cross-sectional study. Int J Surg Open. 2021;28:56–62. doi: https://doi.org/10.1016/j/ijso.2020.12.010
34. Sultana SH, Mawla N, Kawser SH, Akhtar N, Ali MK. Current microbial isolates from wound swab and their susceptibility pattern in a Private Medical College Hospital in Dhaka city. Delta Med Coll J. 2015;3(1):25–30. doi: https://doi.org/10.3329/dmcj.v3i1.22236
35. Mohammed A, Seid ME, Gebrecherkos T, Tiruneh M, Moges F. Bacterial isolates and their antimicrobial susceptibility patterns of wound infections among inpatients and outpatients attending the University of Gondar Referral Hospital, Northwest Ethiopia. Int J Microbiol. 2017;2017:8953829. doi: https://doi.org/10.1155/2017/8953829
36. WHO. Antimicrobial resistance global report on surveillance. 2014. Available from: http://apps.who.int/iris/bitstream/10665/112642/1/978924156478
37. Gelaw A, Gebreselassie S, Tiruneh M, Yifru S, Matiwos E. Isolation of bacterial pathogens from patients with postoperative surgical site infections and possible sources of infections at University of Gondar Hospital, Northwest Ethiopia. J Environ Occup Sci. 2014;3(2):1. doi: https://doi.org/10.5455/jeos.20140512124135
38. Gautam R, Acharya A, Nepal HP, Shrestha S. Antibiotic susceptibility pattern of bacterial isolates from wound infection in Chitwan Medical College Teaching Hospital, Chitwan, Nepal. IJBAR. 2013;4(4):248–52. doi: https://doi.org/10.7439/ijbar.v4i4.302
39. Manikandan C, Amsath A. Antibiotic susceptibility of bacterial strains isolated from wound infection patients in Pattukkottai, Tamil Nadu, India. Int J Curr Microbiol App Sci. 2013;2(6):195–203. Available from: https://ijcmas.com/vol-2-6/C.%20Manikandan%20and%20A.%20Amsath.pdf
40. Nobel FA, Islam S, Babu G, Akter S, Jebin RA, Sarker TC, et al. Isolation of multidrug resistance bacteria from the patients with wound infection and their antibiotics susceptibility patterns: a cross-sectional study. Ann Med Surg. 2022;84:104895. doi: https://doi.org/10.1016/j.amsu.2022.104895
41. Ahmed SMA, Saleh AA, Nigar I, Khan RR, Ahmed S, Sattar ANI, et al. Retrospective study of bacterial profile in wound swab and their susceptibility pattern in a Tertiary Care Hospital in Dhaka, Bangladesh. Arch Microbiol Immunol. 2024;8(3):383–9. doi: https://doi.org/10.26502/ami.936500185
42. Morehead MS, Scarbrough C. Emergence of global antibiotic resistance. Prim Care Clin Off Pract. 2018;45(3):467–84. doi: https://doi.org/10.1016/j.pop.2018.05.006
43. Sutherland R, Croydon EA, Rolinson GN. Amoxicillin: a new semi-synthetic penicillin. Br Med J. 1972;3(5817):13–6. doi: https://doi.org/10.1136/bmj.3.5817.13
44. Keshri V, Panda A, Levasseur A, Rolain JM, Pontarotti P, Raoult D. Phylogenomic analysis of β- lactamase in archaea and bacteria enables the identification of putative new members. Genome Biol Evol. 2018;10(4):1106–14. doi: https://doi.org/10.1093/gbe/evy028
45. Katayama Y, Zhang HZ, Hong D, Chambers HF. Jumping the barrier to β-lactam resistance in Staphylococcus aureus. J Bacteriol. 2003;185(18):5465–72. doi: https://doi.org/10.1128/JB.185.18.5465-5472.2003
46. Yao Q, Gao L, Xu T, Chen Y, Yang X, Han M, et al. Amoxicillin administration regimen and resistance mechanisms of Staphylococcus aureus established in tissue cage infection model. Front Microbiol. 2019;10:1638. doi: https://doi.org/10.3389/fmicb.2019.01638
47. Loeffler A, Pfeiffer DU, Lloyd DH, Smith H, Saoresmagalhaes R, Lindsay JA. Methicillin-resistant Staphylococcus aureus carriage in UK veterinary staff and owners of infected pets: new risk groups. J Hosp Infect. 2010;74(3):282–288. doi: https://doi.org/10.1016/j.jhin.2009.09.020
48. Bhalchandra HM, Naik DS, Verma KP. Aerobic bacterial profile of wound infections and its sensitivity pattern at Tertiary Care Hospital. Int J Curr Microbiol App Sci. 2018;7(6):1668–79. doi: https://doi.org/10.20546/ijcmas.2018.706.198
49. Goswami N, Trivedi HR, Goswami APP. Antibiotic sensitivity profile of bacterial pathogens in postoperative wound infections at a tertiary care hospital in Gujarat, India. J Pharmacol Pharmacother. 2011;2(3):158–64. doi: https://doi.org/10.4103/0976-500X.83279
50. Li L, Dai JX, Xu L, Chen ZH, Li XY, Liu M, et al. Antimicrobial resistance and pathogen distribution in hospitalized burn patients: a multicenter study in Southeast China. Medicine (Baltimore). 2018;97(34):e11977. doi: https://doi.org/10.1097/MD.0000000000011977
51. Sheeba PM, Prathyusha K, Anila MA. Antibiotic susceptibility trends in bacterial isolates from wound infections. MIR J. 2024;11(1):1–9. doi: https://doi.org/10.18527/2024110109
52. Yakha JK, Sharma AR, Dahal N, Lekhak B, Banjara MR. Antibiotic susceptibility pattern of bacterial isolates causing wound infection among the patients visiting B & B hospital. Nepal J Sci Technol. 2015;15(2):91–6. doi: https://doi.org/10.3126/njst.v15i2.12121
53. Gomatheswari SN, Jeyamurugan T. Bacteriological profile and the antibiotic susceptibility pattern of microorganisms isolated from pus/wound swab isolates in patients attending a Tertiary Care Hospital in South India. Int J Curr Microbial App Sci. 2017;6(10):1405–13. doi: https://doi.org/10.20546/ijcmas.2017.610.166
54. Saha AK, Nandi S, Dhar P. Spectrum of microbial isolates from wound infections in patients admitted in a Tertiary Care Hospital, Kolkata. MGM J Med Sci. 2017;4(1):10–8. doi: https://doi.org/10.5005/jp-journals-10036-1130
55. Vikesland P, Garner E, Gupta S, Kang S, Maile-Moskowitz A, Ni Zhu. Differential drivers of antimicrobial resistance across the world. Acc Chem Res. 2019;52(4):916–24. doi: https://doi.org/10.1021/acs.accounts.8b00643
56. Omotayo MA, Avungbeto O, Sokefun OO, Eleyowo OO. Antibacterial activity of Crassocephalum crepidioides (Fireweed) and Chromolaena odorata (Siam weed) hot aqueous leaf extract. Int J Pharm Bio Sci. 2015;5(2):114–22. Available from: https://ijpbs.com/ijpbsadmin/upload/ijpbs_55942e15ef74e.pdf
57. Baral R, Karki A, Karki S, Neupane B, Ko?rala P, Baral S, et al. Phytochemical screening, free radical scavenging, and in vitro anti-bacterial activity studies of various extracts of selected medicinal plants of Nepal. Curr Perspect Med Aroma Plant. 2021;4(1):22–35. doi: https://doi.org/10.38093/cupmap.896273
58. Huang J, Zaynab M, Sharif Y, Khan J, Al-Yahyai R, Sadder M, et al. Tannins as antimicrobial agents: understanding toxic effects on pathogens. Toxicon. 2024;247:107812. doi: https://doi.org/10.1016/j.toxicon.2024.107812
59. Begashaw B, Mishra B, Tsegaw A, Shewamene Z. Methanol leaves extract Hibiscus micranthus Linn exhibited antibacterial and wound healing activities. BMC Complement Altern Med. 2017;17(1):337. doi: https://doi.org/10.1186/s12906-017-1841-x
60. Gupta PD, Birdi TJ. Development of botanicals to combat antibiotic resistance. J Ayurveda Integr Med. 2017;8(4):266–75. doi: https://doi.org/10.1016/j.jaim.2017.05.004
61. Wirahmi N, Masrijal CDP, Amri F, Ikhsan, Triyansyah MI. Formulation and antibacterial activity of natural disinfectant combination of Psidium guajava and piper betle leaf infusion against Staphylococcus aureus. Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021). Atlantis Pressp. 91–7. doi: https://doi.org/10.2991/ahsr.k.211105.013
62. Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial activity of polyphenols and alkaloids in middle eastern plants. Front Microbiol. 2019;10:911. doi: https://doi.org/10.3389/fmicb.2019.00911
63. Saxena D, Maitra R, Bormon R, Czekanska M, Meiers J, Titz J, et al. Tackling the outer membrane: facilitating compound entry into Gram-negative bacterial pathogens. Antimicrob Resist. 2023;1:17. doi: https://doi.org/10.1038/s44259-023-00016-1
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