Java Sea Spirulina platensis chemical analysis and its protective ability against H2O2-exposed umbilical cord mesenchymal stem cells according to CD73, CD90, and CD105, viability, and HIF-1 alpha docking

Ani Retno Prijanti Retno Wahyu Nurhayati Febriana Catur Iswanti Zakiyatul Khoiriyah Reni Paramita Fadilah Fadilah   

Open Access   

Published:  Jun 13, 2022


Background: It is known that Spirulina has so many healing functions to overcome oxidative stresses correlated to the aging process. One of them is Spirulina platensis (SP), a microalga that is abundant in the Java Indonesian seas and has been widely cultivated. SP is able to improve mitochondrial function and is widely used as a food supplement according to its protein and content of omega-6, gamma linolenic acid, carotenoid, and various vitamins. This research was conducted to evaluate the effect of SP on cell viability and used a mesenchymal stem cell (MSC) culture as a model treated with hydrogen peroxide together with SP.

Methods: SP was obtained from the Java Sea and extracted with ethanol. Its content was determined with the thin layer chromatography method, and the known content was docked with the HIF-1 protein. This extract was tested to overcome oxidative stress in umbilical cord-derived mesenchymal stem cells (UC-MSCs) treated with 100 and 300 µM hydrogen peroxide. Cell viability was assessed by a dye exclusion method with trypan blue. The specific surface markers (CD73, CD90, and CD105) were measured with flow cytometry to evaluate MSC stemness.

Result: The content of SP, phycocyanobilin and canthaxanthin, were considered candidates affecting HIF-1 protein regulation. The SP ethanol extract (125 ng/ml) maintained the viability and stemness of UC-MSCs during oxidative stress exposure with hydrogen peroxide.

Conclusion: The phycocyanobilin and canthaxanthin of SP were considered candidate substances that could affect HIF-1 signaling, and the ability to overcome oxidative stress should be observed further.

Keyword:     Spirulina platensis MSC CD70 CD90 CD105 HIF-1 alpha.


Prijanti AR, Nurhayati RW, Iswanti FC, Khoiriyah Z, Paramita R, Fadilah F. Java Sea Spirulina platensis chemical analysis and its protective ability against H2 O2 -exposed umbilical cord mesenchymal stem cells according to CD73, CD90, and CD105, viability, and HIF-1 alpha docking. J Appl Pharm Sci, 2022. Online First.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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