Quantitative computation and stability evaluation of phase III composition comprising sitagliptin and dapagliflozin propanediol monohydrate by RP-HPLC

Instruments and chemicals

AGILENT (Series 1260 infinity) HPLC unit equipped with autosampler having provision of UV-VIS detector and capable of quaternary gradient operation was used to process the samples, while OPENLAB sofftware was used to process and integrate the obtained chromatogram. The columns employed were INERTSIL ODS C₁₈ and HIBAR ODS C₁₈ having dimensions of 250 mm as length and 4.6 mm internal diameter. Mettler Toledo weighing balance was used to weigh accurate quantities of samples having sensitivity of 0.1 mg.

Both active components were received as gift samples from Cadila Healthcare Limited, Gujarat (Batch No: SA0010119 for SITA and GLI500119 for DAPA) while other solvents like Methyl alchohol, methyl nitrile, KH₂PO₄ buffer and water were of HPLC grade and procured from Merck life scieces private limited, Mumbai.

Optimization of separation conditions

Discrite intrinsic physico-chemical parameters of active components were studied but atmost priority was given to solubility and pKa value. Other instrumental parameters like flow rate and temperate were also studied to check effect on retention time of eluting components. Owning to the vast difference in polarity of components, ODS C₁₈ column was fixed as stationary phase which encourages the retention of nonpolar and early elution of polar component. To get adequate separation in conjuction with system suitability parameters, various elution systems were studied.

Chromatographic conditions

Adequate separation was achived by utilizing methyl nitrile as organic phase in 25 parts and 75 parts of 0.02 M potassium dihydrogen phosphate buffer containing 1 ml triethylamine (TEA) having neutral pH adjusted by orthophosphoric acid (10%) as aqueous phase. The mixed elution system was screened through 0.45 µ membrane filter and was also utilized as dilution medium for components. The above-methioned elution system was allowed flow though INERTSIL ODS C18 column in isocratic mode at flow rate of 1 ml/minute with monitoring on 210 nm as wavelength where both components gave significant signal. Futhermore, suitability of this system was also assesed by system suitability parameters for which total five injections of fixed concentration was injected at 20 µl injector volume.

Forced degradation conditions

Acid and base hydrolysis

To check the stability with respect to hydrolysis by acidic condition, 5 mg of DAPA and 50 mg of SITA was tretaed with 5 ml of 0.1 N HCl and 4 ml of elution medium, the resulting mixture was refluxed at 60°C for half and hour which was followed by cooling of content and neutralization with 0.1 N NaOH. 0.2 ml of previous solution was further diluted to 10 ml with elution medium (Treated sample). In contrast, for base hydrolysis, 0.1 N NaOH was used as the treatment medium and 0.1 N HCl was used as the neutralizing medium. All other condition were same as that of acid hydrolysis. To compare the results, 0 hour sample and blank was prepared. 20 µl of 0 hour sample and treated sample was injected on to column and comparison was made to determined % degradation.

Oxidative hydrolysis

Effect of oxidative stress was imparted to both drug by use of 3% hydrogen peroxide. Here in this case, 5 mg of DAPA and 50 mg of SITA was tretaed with 5 ml of 3% H₂O₂ and 4 ml of elution medium, the resulting mixture was refluxed at 60°C for half and hour which was followed by cooling and again volume was adjusted upto the mark. 0.2 ml of the above solution was further diluted to 10 ml with elution medium (treated sample). In similar way, 0 hour sample and blank solution was prepared and were injected on to column to assess the % degrdation achieved.

Thermal hydrolysis

To asses impact of dry heat on the stability of DAPA and SITA, both componets in preweight quatity (10 and 100 mg, respectively) were exposed to dry heat in a hot air oven at 80°C for about half hour. Treated contents were diluted upto 10 ml with mobile phase and 0.1 ml of same solution was further raised to 10 ml which was injected at 20 µl volume on to column to assess % degradation.

Validation of optimized method

All validation parameters are studies as per ICH Q2R1 guidelines, execution procedure of each is discussed below.

Linearity and range

To study linearity and range, accurately weighed quantities of 5 mg DAPA and 50 mg SITA was diluted to 10 ml with elution medium to produce stock solution containing 500 µg/ml DAPA and 5,000 µg/ml of SITA. Various aliquotes were transferred to 10 ml volumetric flask and volume of each raised to 10 ml to give mixtures having concentration of 5 + 50 µg/ml to 15 + 150 µg/ml of DAPA and SITA, respectively. Each concentration was injected at 20 µl injector volume and response obtained was plotted against concentration to observe linear regression coefficient.

Repeatability

For adjudging repeatability of method, each concentration form range was injected for five times and each level is observed for relative standard deviation (RSD).

Limit of detection and quantitation (LOD/LOQ)

Detection limit as well as qunatification limit was calculated statistically by utlization of regression data from repeatability. Standard devidation of standard error (σ) and average of slope (S) were used to process LOD and LOQ.

Accuracy

As it refers to the % recovery of analyte in presence of exipients, it was practiced by spiking of placebo with standard at 50%, 100%, and 150% of target concentration. Each concentration was injected for three times and % recovery was calculated on the basis of onserved area at each spiking level (by utilization of linear regression analysis).

Method precision

This parameter was studies by injecting individual concentration that represents overall range are studies on same day and between days and for the same mixture of DAPA and SITA that represents overall range (8 + 2, 16 + 4, and 24 + 6 µg/ml) were analyzed on the same day at different time interval for intraday precision and different day for interday precision. Each oncentration was analyzed for three times and was monitored for RSD at each level.

Robustness

To study the effect of minor variations of method parameters on to the results, operation parameters like temperature, pH of mobile phase and flow rate was delibrately altered within acceptable range and effect of the same was observed on the system suitability parameters as retention time and % assay, which was also monitored for relative standard devidation.

Ruggedness

This parameters refers to the method repeatability and was studied by utilizing the diffent column having same specification and to be more precise HIBAR ODS C₁₈ column was employed and difference in the area obtained from both was monitored through student t-test.

Assay of synthetic mixture

Weight accurately 10 mg of DAPA, 100 mg of SITA with placebo in 10 ml calibrated volumetric flask. Contents diluted up to 10 ml calibrated volumetric flask with methyl alcohol and filter the solution. 1 ml of master stock solution further dilute up to 10 ml with mobile phase. 1 ml filtrate diluted up to 10 ml with mobile phase (10 + 100 μg/ml). Resulting solution was chromatographed in triplicates and mean observed area was statistically transformed by linear regression equation to get toal % of DATA and SITA in synthetic mixture.

Optimized chromatographic conditions

Initially trials were initiated using combination of methyl nitrile and water in equal proportion but distroted peak without any sort of separation were observed. As separation was not observed in the previous case, methyl alcohol was introduced in to the system but with the same only DAPA was detectd. In all the cases, flow rate was 1.2 ml/minute. All the results were monitored at wavelengths as 210, 224, and 267 nm. Due to previous observed problems, 0.02 M KH₂PO4 (0.02 M) containing 1 ml TEA and pH adjusting to 7 by orthophosphoric acid in equal volumes was used as elution medium. Here, both the peak were detected but still were eluting closely to each other. So in the next trial the proportion of buffer was increased and flow rate was further reduced to 1 ml/minute. Even the solution of DAPA and SITA were also prepared in the mobile phase. When method was operated using later discussed chromatographic conditions, well resolved peak of DAPA and SITA was observed at 2.89 and 16.41 minutes, respectively (Fig. 1). The detection wavelength was kept at 210 nm as linearity and peak shape for both the components were perfact at this wavelength. All the system suitability paramerets were within United States Pharmacopoeia guidelines with RSD of less than 1. All the system suitability paramerets have been highlighted in Table 1. The newly developed method was found to be superior to reported method (Gupta and Mishra, 2021) by far degree as they had employed gradient elution with potassium phosphate monobasic buffer pH (3.0) as mobile phase A while methanol and acetonitrile in the ratio of (60:40 v/v) as a mobile phase B. The condition its self is too complex and the flow of elution was kept at 1.5 ml/minute which gives rise to too much column back pressure. Furthermore, the major con of the reported method is that, both DAPA and SITA have the same concentration range, which is quite contrary to the fixed combination dose, and hence the newly developed method outsmarts the reported method in all the segments.

Figure 1. Chromatogram of mixture of DAPA and SITA under optimized chromatographic conditions. [Click here to view]

Table 1. System suitability parameters for optimized RP-HPLC method. [Click here to view]

Forced degradation study

When optimized method was apllied for evaluating the stability and behaviour of active components by forced degradation study (acid hydrolysis, base hydrolysis, oxidative stress, and thermal stress), the optimized method was found to be stability indicating as it is able to separate all the degradation products in the presence of active ingridients. No degradation products found to interfere with estimation of DAPA and SITA in stressed samples (Figs. 2–4). Even the stress condition applied for the study were found to be optimim as, % degradation observed was predictive in nature (below 20%). % degradation observed for each condition is highlighted in Table 2.

Validation of analytical method

The proposed optimized RP-HPLC method was studied for validation parameters according to the ICH Q2R1 guidelines and each validation parameter was found to be in accordance with guidelines. Method was found to be linear with linear regression co-efficient of 0.9983 and 0.999 over the range of 5–15 µg/ml of DAPA and 50–150 µg/ml SITA, respectively (Figs. 5–7). Method was found to be robust and rugged as there is no significant change in results (retention time and % assay) by minor variation in method parameters, the same highlighted in Tables 3 and 4. Summary of all validation parameters is highlighted in Table 5. When optimized and validated method was applied for quantitative analysis of synthetic mixture containing DAPA and SITA (Fig. 8), the method gave accurate results with % of DAPA and SITA as 99.43 and 99.41, respectively.

Figure 2. Chromatogram of treated sample with 0.1 N Hydrochloric acid (acid hydrolysis). [Click here to view]

Figure 3. Chromatogram of treated sample with 0.1 N NaOH (base hydrolysis). [Click here to view]

Figure 4. Chromatogram of treated sample with 3% hydrogen peroxide (oxidative stress). [Click here to view]

Table 2. Summary of forced degradation studies. [Click here to view]

Figure 5. Overlain chromatogram of mixtures representative of linearity study. [Click here to view]

Figure 6. Regression analysis of DAPA (For linearity studies). [Click here to view]

Figure 7. Regression analysis of SITA (for linearity studies). [Click here to view]

Table 3. Robustness data of optimized RP-HPLC method. [Click here to view]

Table 4. Ruggedness of the developed RP-HPLC method. [Click here to view]

Table 5. Summary of validation parameters. [Click here to view]

Figure 8. Assay of synthetic mixture (DAPA and SITA, 10 and 100 μg/ml, respectively). [Click here to view]