Research Article | Volume: 8, Issue: 11, November, 2018

Evaluation of anticonvulsant effects of stem bark of Anogeissus latifolia (Roxb.) in mice

Vikas Chandra Sharma Atul Kaushik   

Open Access   

Published:  Nov 30, 2018

DOI: 10.7324/JAPS.2018.81110

The stem bark of Anogeissus latifolia (Roxb.) (Family: Combretaceae) is used for the correction of various ailments in the traditional system of medicine. The present study aimed to investigate the anticonvulsant effect of the stem bark of A. latifolia (Roxb.). Anticonvulsant effect was assessed in maximal electroshock (MES) and pentylenetetrazol (PTZ)-induced convulsions in mice. Electric shock (50 mA for 0.2 seconds) was delivered with the help of corneal electrode to induce hind limb tonic extension, while pentylenetetrazol (80 mg/kg) was injected intraperitoneally to induce convulsion (clonic). Different concentrations of ethanolic extract of stem bark of A. latifolia (200, 400, and 600 mg/kg) were administered orally to mice. The ethanolic extract of stem bark of A. latifolia showed significant dose-dependent protection against seizures in both MES and PTZ-induced convulsion models. These findings suggested that ethanolic extract of stem bark of A. latifolia exhibited anticonvulsant effect against MES and PTZ-induced convulsions in a dose-dependent manner which may be attributed to the presence of ellagic acid and other tannins.

Keyword:     Dhava seizures phenytoin ellagic acid pentylenetetrazol.


Sharma VC, Kaushik A. Evaluation of anticonvulsant effects of stem bark of Anogeissus latifolia (Roxb.) in mice. J App Pharm Sci, 2018; 8(11): 069–074.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bala LD, Singh R. Traditional use of herbal medicines against Rheumatism by the tribals of Satna District (M.P.). Int J Sci Res, 2013; 4:1259–64.

Bala LD, Singh R. Ethnobotanical study of traditional medicinal plants used by Mawasi tribe of Chitrakoot region Distt. Satna (M.P.) India. Int J Biol Res, 2016; 1:13–5.

Browning R. The electro shock model, neuronal network and antiepileptic drugs. In: Faingold CL, Fromm GH (eds). Drugs for control of epilepsy: actions on neuronal networks in seizure disorders. CRC Press, Boca Raton, FL, 1992.

Czapinski P, Blaszczyk B, Czuczwar SJ. Mechanisms of action of antiepileptic drugs. Curr Top Med Chem, 2005; 5:3–14.

De Sarro G, Ferreri G, Gareri P, Russo E, De Sarro A, Gitto R, Chimirri A. Comparatie antionvulsant activityof some 2, 3-benzodiazepine derivaties in rodents. Pharmacol Biochem Behav, 2003; 74:595–602.

Deshpande VH, Patil AD, Rama Rao AV, Venkatraman K. 3, 3'-Di- O-methyl ellagic acid-4'-β-D-Xyloside and 3, 4, 3'-tri-O-methylflavellagic acid-4'-β-Dglucoside from the Anogeissus latifolia bark. Indian J Chem, 1976; 14:641–3.

Dhingra D, Jangra A. Antiepileptic activity of ellagic acid, a naturally occurring polyphenolic compound, in mice. J Funct Foods, 2014; 10:364–9.

Gasior M, Carter RB, Goldberg SR, Witkin JM. Anticonvulsant and behavioral effects of neuroactive steroids alone and in conjunction with diazepam. J Pharmacol Exp Ther, 1997; 282:543–53.

Govindrajan R, Vijaykumar M, Rao CV, Shirwaikar A, Mehrotra S, Pushpangadan P. Healing potential of Anogeissus latifolia for dermal wounds in rats. Acta Pharm, 2004a; 54:331–8.

Govindrajan R, Vijaykumar M, Rao CV, Shirwaikar A, Rawat AKS, Mehrotra S, Pushpangadan P. Antioxidant potential of Anogenissus latifolia. Biol Pharm Bull, 2004b; 27:1266–9.

Govindrajan R, Vijaykumar M, Singh M, Rao CV, Shirwaikar A, Rawat AKS, Rawat P, Pushpangadan P. Antiulcer and antimicrobial activity of Anogeissus latifolia. J Ethnopharmacol, 2006; 106:57–61.

Jain SK. Dictionary of Indian folk medicine and ethnobotany. Deep publication, New Delhi, India, 1991.

Jain SP, Singh SC, Srivastava S, Singh J, Mishra NP, Prakash A. Hitherto unreported ethnomedicinal uses of plants of Betul district of Madhya Pradesh. Indian J Trad Knowl, 2010; 9:522–5.

Kabuto H, Yokoi I, Mori A. Monoamine metabolites, iron induced seizures, and the anticonvulsant effect of tannins. Neurochem Res, 1992; 17:585–90.

Khandelwal KR. Practical pharmacognosy techniques and experiments. 9th edition, Nirali Prakashan, New Delhi, India, 2002.

Kirtikar KR, Basu BD. Indian medicinal plants. Jayad Press, New Delhi, India, 1975.

Kohling R. Voltage-gated sodium channel in epilepsy. Epilepsia, 2002; 43:1278–95.

Kulkarni SK. Hand book of experimental pharmacology. 3rd edition, Vallabh Prakashan, New Delhi, India, 1999.

Liow K, Barkley GL, Pollard JR, Harden CL, Bazil CW. Position statement on the coverage of anticonvulsant drugs for the treatment of epilepsy. Neurology, 2007; 68:1249–50.

Löscher W, Schmidt D. Which animal models should be used in the search for new antiepileptic drugs? A proposal based on experimental and clinical considerations. Epilesy Res, 1988; 2:145–81.

Nag D. Gender and epilepsy: a Clinician's experience. Neurol Ind, 2000; 48:99–104.

Nag, A. Galav P, Kateva SS. Indigenous animal health practices from the Udaipur district, Rajasthan. Indian J Trad Knowl, 2007; 6:583–8.

Nisar M, Khan I, Simjee SU, Gilani AH, Obaidullah, Perveen H. Anticonvulsant, analgesic and antipyretic activities of Taxus wallichiana Zucc. J Ethnopharmacol, 2008; 116:490–4.

Organization for Economic Cooperation and Development (OECD). Guideline for the testing of chemicals, Revised draft guideline 423. Document on acute oral toxicity and acute toxicity class method, 2001. Available via (Accessed 21 June 2013).

Parvathi KMM, Ramesh CK, Krishna V, Paramesha M, Kuppast IJ. Hypolipidemic activity of gum ghatti of Anogeissus latifolia. Pharmacogn Mag, 2009; 5:11–4.

Patil MV, Patil DA. Ethnomedicinal practices of Nasik district, Maharashtra. Indian J Trad Knowl, 2005; 4:287–90.

Pawar S, Patil DA. Ethnobotany of Jalgoan district, Maharashtra. Daya publishing House, New Delhi, India, 2008.

Pradeep HA, Khan S, Ravikumar K, Ahmed MF, Rao MS, Kiranmai M, Reddy DS, Ahamed SR, Ibrahim M. Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies. World J Gastroenterol, 2009; 15:4816–22.

Rahman MS, Rahman MZ, Ahad Uddin ABM, Rashid MA. Steriod and triterpenoid from Anogeissus latifolia. Dhaka Univ J Pharm Sci, 2007; 6:47–50.

Ramachandran S, Naveen KR, Rajinikanth B, Akbar M, Rajasekaran A. Antidiabetic, antihyperlipidemic and in vivo antioxidant potential of aqueous extract of Anogeissus latifolia bark in type 2 diabetic rats. Asian Pac J Trop Dis, 2012; 2:S596–602.

Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology. 7th edition, Churchill and Livingstone, London, 2012.

Reddy KK, Rajadurai S, Nayudamma Y. Studies on Dhava (Anogeissus latifolia) Tannins: Part III-polyphenols of bark, sap-wood and heart wood of Dhava. Indian J Chem, 1965; 3:308–10.

Reddy KK, Rajadurai S, Sastry KNS, Nayudamma Y. Studies on dhava tannins. I. Dhava tannins. The isolation and constitution of a gallotannin from dhava (Anogeissus latifolia). Aust J Chem, 1964; 17:238–45.

Rho JM, Sankar R. The pharmacologic basis of antiepileptic drug action. Epilepsia, 1999; 40:1471–83.

Rogawski MA, Porter RJ. Antiepiletic drugs: pharmacological mechanisms and clinical efficacy with consideration of promising developmental stage compounds. Pharmacol Rev, 1990; 42:223–86.

Saeed S, Mosa-Al-Reza H, Fatemeh AN, Saeideh D. Antihyperglycemic and antihyperlipidemic effects of guar gum on streptozotocin-induced diabetes in male rats. Pharmacogn Mag, 2012; 8:65–72.

Samar R, Shrivastava PN, Jain M. Ethnobotanical study of traditional medicinal plants used by tribe of Guna district, Madhya Pradesh, India. Int J Curr Microbiol App Sci, 2015; 4:466–71.

Singh D, Baghel US, Gautam A, Baghel DS, Yadav D, Malik J, Yadav R. The genus Anogeissus: A review on ethnopharmacology, phytochemistry and pharmacology. J Ethnopharmacol, 2016; 194:30–56.

Stahl I. Thin layer chromatography. A laboratory hand book. Springer-Verlag, Berlin, Germany, 1969.

Swinyard EA, Brown WC, Goodman LS. Comparative assays of antiepileptic drugs in mice and rats. J Pharmacol Exp Ther, 1952; 106:319–30.

Venkata Ratnam K, Venkata Raju RR. Folk remedies for insect bite from Gundlabrahmeswaram wild life sanctuary, Andra Pradesh. Indian J Trad Knowl, 2008; 7:436–7.

Yokoi I, Kabuto H, Akiyama K, Mori A, Ozaki M. Tannins inhibit the occurrence of epileptic focus induced by FeCI3, injection in rats. Jpn J Psychiatry Neurol, 1989; 43:552–3.

Zhang ZJ. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci, 2004; 75:1659–99.

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