Research Article | Volume: 8, Issue: 11, November, 2018

Dried flower powder combination of Clitoria ternatea and Punica granatum demonstrated analogous anti-hyperglycemic potential as compared with standard drug metformin: In vivo study in Sprague Dawley rats

Sachin P. Borikar Nilesh G. Kallewar Debarshi Kar Mahapatra Nitin G. Dumore   

Open Access   

Published:  Nov 30, 2018

DOI: 10.7324/JAPS.2018.81111

The diabetes mellitus type-2 (DMT-2) is among the highly escalating disease that is expected to affect nearly 400 million people across the globe by the end of 2030. In DMT-2, the control of post-prandial surge is the most imperative requirement for the management of metabolic disturbances. The inclined trend toward herbal-based medicine among the developing nations owing to their safety and reduced side-effects has compelled the researchers to explore the hidden potentials of herbs and shrubs of Mother Nature. The increasing interest of herbal products among the patient population has also revolutionized the traditional ethnopharmacological aspects. While processing toward the direction, the research involved yet unreported anti-hyperglycemic activity of two well-known Indian plants Clitoria ternatea (Family: Fabaceae) and Punica granatum (Family: Punicaceae). In the present study, the dried flower powder of C. ternatea and P. granatum was screened individually and in combination for their anti-hyperglycemic activity in alloxan-induced diabetic rat model employing the standard drug metformin hydrochloride. The research demonstrated that the combination of dried flower powder of C. ternatea and P. granatum exhibited a significant anti-hyperglycemic effect after 5 hours of oral administration. The phytoconstituents present in the plant material; primarily, the flavonoids and alkaloids play a crucial role in mediating the pharmacological activity. This study will open new perspectives of future research in developing novel herbal formulations for the management of diabetes mellitus at the clinical level.

Keyword:     Hypoglycemic Clitoria ternatea Punica granatum flower powder alloxan.


Borikar SP, Kallewar NG, Mahapatra DK, Dumore NG. Dried flower powder combination of Clitoria ternatea and Punica granatum demonstrated analogous anti-hyperglycemic potential as compared with standard drug metformin: In vivo study in Sprague Dawley rats. J App Pharm Sci, 2018; 8(11): 075–079.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Askary-Ashtiani A, Ghanjal A, Motaqi M, Meftahi GH, Hatef B, Niknam H. The isokinetic and electromyographic assessment of knee muscles strength in the short-and long-term type 2 diabetes. Asian J Sport Med, 2016; 7(4):e37008.

Bagri P, Ali M, Aeri V, Bhowmik M, Sultana S. Antidiabetic effect of Punica granatum flowers: effect on hyperlipidemia, pancreatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes. Food Chem Toxicol, 2009; 47(1):50–4.

Borikar SP, Kallewar NG, Mahapatra DK, Dumore NG, Danao KR. Combined flower decoction of Clitoria ternatea and Punica granatum expressed comparable hypoglycemic activity with that of metformin: in vivo study. Int J Pharm Science Res, 2017; 2(5):4–7.

Borikar SP, Kallewar NG, Mahapatra DK, Gupta RA, Dumore NG, Danao KR, et al. Decoction of Andrographis paniculata whole plant and Gymnema sylvestre leaves demonstrated noteworthy hypoglycemic activity in Sprague Dawley rat. Phytopharmacol J, 2018; 7(1):84–7.

Chhajed SS, Chaskar S, Kshirsagar SK, Haldar GA, Mahapatra DK. Rational design and synthesis of some PPAR-γ agonists: substituted benzylideneamino-benzylidene-thiazolidine-2, 4-diones. Comp Biol Chem, 2017; 67:260–5.

Daisy P, Rajathi M. Hypoglycemic effects of Clitoria ternatea Linn. (Fabaceae) in alloxan-induced diabetes in rats. Trop J Pharm Res, 2009; 8(5):393–8.

Daisy P, Santosh K, Rajathi M. Antihyperglycemic and antihyperlipidemic effects of Clitoria ternatea Linn. in alloxan-induced diabetic rats. Afr J Microbiol Res, 2009; 3(5):287–91.

Das S, Barman S. Antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of Punica granatum in alloxan-induced non– insulin-dependent diabetes mellitus albino rats. Indian J Pharmacol, 2012; 44(2):219–24.

Das AK, Mandal SC, Banerjee SK, Sinha S, Saha BP, Pal M. Studies on the hypoglycaemic activity of Punica granatum seed in streptozotocin induced diabetic rats. Phytother Res, 2001; 15(7):628–9.

Godbole MD, Mahapatra DK, Khode PD. Fabrication and characterization of edible jelly formulation of stevioside: a nutraceutical or OTC aid for the diabetic patients. Inventi Nutraceut, 2017; 2017(2):1–9.

Huang TH, Peng G, Kota BP, Li GQ, Yamahara J, Roufogalis BD, Li Y. Anti-diabetic action of Punica granatum flower extract: activation of PPAR-γ and identification of an active component. Toxicol Appl Pharmacol, 2005; 207(2):160–9.

Jafri MA, Aslam M, Javed K, Singh S. Effect of Punica granatum Linn.(flowers) on blood glucose level in normal and alloxan-induced diabetic rats. J Ethnopharmacol, 2000; 70(3):309–14.

Kalyan BV, Kothandam H, Palaniyappan V, Praveen AR. Hypoglycaemic activity of seed extract of Clitoria ternatea Linn in streptozotocin-induced diabetic rats. Pharmacognosy J, 2011; 3:45–7.

Kamble MA, Mahapatra DK, Dhabarde DM, Ingole AR. Pharmacognostic and pharmacological studies of Bombax ceiba thorn extract. J Pharm Pharmacog Res, 2017; 5(1):40–54.

Kanhed AA, Mehere AP, Pandey KR, Mahapatra DK. 4-(2-chloroacetamido) Benzoic acid derivatives as local anesthetic agents: design, synthesis, and characterization. UK J Pharm Biosci, 2016; 4(6):35–44.

Kavitha R, Premalakshmi V. Studies on the synergetic effect of Trichosanthes dioica and Clitoria ternatea leaf extract on the streptozotocin-induced diabetic rats. Int J Res Pharm Biomed Sci, 2012; 3(3):1056–64.

Khalil EA. Antidiabetic effect of an aqueous extract of Pomegranate (Punica granatum L.) peels in normal and alloxan diabetic rats. Egyptian J Hospital Med, 2004; 16(1):92–9.

Kuhite NG, Padole CD, Amdare MD, Jogdand KR, Kathane LL, Mahapatra DK. Hippuric acid as the template material for the synthesis of a novel anti-diabetic 1,3,4-thiadiazole derivative. Indian J Pharm Biol Res, 2017; 5(3):42–5.

Li Y, Wen S, Kota BP, Peng G, Li GQ, Yamahara J, et al. Punica granatum flower extract, a potent α-glucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats. J Ethnopharmacol, 2005; 99(2):239–44.

Mahapatra DK, Asati V, Bharti SK, Chalcones and their therapeutic targets for the management of diabetes: structural and pharmacological perspectives. Eur J Med Chem, 2015; 92:839–65.

Mahapatra DK, Bharti SK. Drug design. Tara Publications Private Limited, New Delhi, India, 2016.

Mahapatra DK, Bharti SK. Handbook of research on medicinal chemistry: innovations and methodologies. Apple Academic Press, New Jersey, 2017.

Mahapatra DK, Bharti SK. Medicinal chemistry with pharmaceutical product development. Apple Academic Press, New Jersey, 2019.

Mahapatra DK, Chhajed SS, Shivhare RS. Development of murrayanine-chalcone hybrids: an effort to combine two privilege scaffolds for enhancing hypoglycemic activity. Int J Pharm Chem Anal, 2017; 4(2):30–4.

Radhika S, Smila KH, Muthezhilan R. Antidiabetic and hypolipidemic activity of Punica granatum Linn on alloxan induced rats. World J Med Sci, 2011; 6(4):178–82.

Sharma AK, Majumder M. Some observations on the effect of Clitoria Ternatea Linn on changes in serum sugar level and small intestinal mucosal carbohydrases activities in alloxan diabetes. Calcutta Med J, 1990; 87(11–12):168–71.

Talpate KA, Bhosale UA, Zambare MR, Somani R. Antihyperglycemic and antioxidant activity of Clitorea ternatea Linn. on streptozotocin-induced diabetic rats. Ayu, 2013; 34(4):433–39.

Verma PR, Itankar PR, Arora SK. Evaluation of antidiabetic antihyperlipidemic and pancreatic regeneration, potential of aerial parts of Clitoria ternatea. Rev Bras Farmacogn, 2013; 23(5):819–29.

Article Metrics
525 Views 131 Downloads 656 Total



Related Search

By author names