Research Article | Volume: 8, Issue: 8, August, 2018

Dendrophthoe pentandra Leaves Extract Promotes Apoptotic Effects of Doxorubicin in Human Breast Cancer Cell via Modulation of Intracellular Calcium and Survivin

Agustina Tri Endharti Tri Esti Wahyuningtyas Hardini Kusworini Handono Edi Widjajanto Sofy Permana   

Open Access   

Published:  Aug 31, 2018

DOI: 10.7324/JAPS.2018.8806

Dendrophthoe pentandra is a parasitic plant that has anticancer activity. The leaf of Dendrophthoe pentandra traditionally has been used for anticancer agents. The aim of this study was to investigate whether the combination of Dendrophthoe pentandra leaves extract (DPE) and doxorubicin reduces intracellular calcium concentration [iCa2+] and survivin levels while increasing the number of apoptotic cells in MCF-7 breast cancer cell lines. MCF-7 cells were cultured and divided into six groups: group 1 is a negative control (without DPE nor doxorubicin); group 2 was treated with doxorubicin alone; groups 3-5 were treated with DPE in doses of either 12.5, 25, or 50 μg/ml immediately after doxorubicin treatment, and group 6 was treated with 50 μg/ml of DPE alone. After 24 hours of treatment, we assessed the concentration of iCa2+, survivin levels and the number of apoptotic cells by flow cytometry. The combination of doxorubicin and DPE significantly reduced the percentage of survivin (P < 0.05) and iCa2+ concentration (P < 0.05); whereas the number of apoptotic cells increased significantly (P < 0.05). Therefore, we concluded that the anti-cancer effect of doxorubicin can be enhanced when combined with DPE.

Keyword:     Apoptosis intracellular calcium Dendrophthoe pentandra extract doxorubicin survivin.


Endharti AT, Wahyuningtyas TE, Hardini, Handono K, Widjajanto E, Permana S. Dendrophthoe pentandra Leaves Extract Promotes Apoptotic Effects of Doxorubicin in Human Breast Cancer Cell via Modulation of Intracellular Calcium and Survivin. J App Pharm Sci, 2018; 8(08): 039-043.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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