Research Article | Volume: 8, Issue: 7, July, 2018

Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression

Agustina Tri Endharti ,  Eni Sulastri,  Rabiah Umanailo,  Yunialce,  Tatit Nurseta,  Kusworini Handono   
Agustina Tri Endharti1 & 2, Eni Sulastri3, Rabiah Umanailo3, Yunialce3, Tatit Nurseta4, Kusworini Handono5

1Department of Parasitology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.

2Biomedical Central Laboratory, Faculty of Medicine, Brawijaya University, Indonesia.

3Master Program in Midwifery, Faculty of Medicine, Brawijaya University, Malang, Indonesia.

4Departement of Gynecology and Onco-gynecology Saiful Anwar Hospital, Medical Faculty, University of Brawijaya, Malang, East Java, Indonesia.

5Department of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.

Open Access   

Published:  Jul 30, 2018

DOI: 10.7324/JAPS.2018.8702
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Abstract

Mango mistletoe (Dendrophthoe pentandra L. Miq) (MMDP) leaf extract which contained quercetin inhibits cancer cells. MMDP increasing effectively of 5-Fluorouracil (5-FU) as a chemotherapy agent. This study aimed to investigate the synergistic effects of MMDP combined with 5-FU to determine the percentage of apoptosis, p21, and survivin in HeLa cells. Flow cytometry analysis revealed that it increases apoptosis HeLa cell through increasing p21 and also decreased survivin percentage. The combination of 5-FU 5 μg/ml and MMDP 50 μg/ml significantly increased the percentage of p21 and apoptosis (P < 0.001; P < 0.001) and decreased survivin (P < 0.05) compared with 5-FU only group. This study indicates that the combined 5-FU and MMDP has a synergistic effect to enhance apoptosis and p21 expressions by decreasing of survivin in HeLa cells.


Keyword:     Apoptosis 5-FU Mango mistletoe (Dendrophthoe pentandra) p21 survivin.

Citation:

Endharti AT, Sulastri E, Umanailo R, Yunialce, Nurseta T, Handono K. Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression. J App Pharm Sci, 2018; 8(07): 010-015.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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