A new research method has been developed to approach multiresponse optimization for simultaneously optimizing a large number of experimental factors. LC Chromatogram was optimized using Phenomenex RP C18 column (250 x 4.6 mm; 5 μm); mobile phase was surged at isocratic mode with a flow of 1.0 mL/min using methanol and acetonitrile (95:5% v/v) at the detection max of 208 nm with the retention time of 16.3 and 18.1 min for Stigmasterol and β-Sitosterol respectively. Amount of Stigmasterol and β-Sitosterol was quantified and found to be 51.0 and 56.3 μg/ mg respectively. The r2 value of 0.9971 and 0.9960 was found in the linear range of 80-130 μg/mL for Stigmasterol and β-Sitosterol respectively. LOD and LOQ were 6.951, 21.063 μg/mL and 6.048, 18.328 μg/mL for Stigmasterol and β-Sitosterol respectively. The relative standard deviation for the system and the method precision were found to be 0.94%, 0.40% and 1.51%, 1.1% (≤2%) for stigmasterol and β-Sitosterol. Recovery studies were performed and found in the range of 95-105% indicates the accuracy of the developed method. The developed chromatographic method is the first report for the concurrent estimation of Stigmasterol and β-Sitosterol in Manasamitra Vatakam.
Vemuri S, Ramasamy MK, Rajakanu P, Kumar RCS, Kalliappan I. Application of Chemometrics for the simultaneous estimation of stigmasterol and β-sitosterol in Manasamitra Vatakam-an ayurvedic herbomineral formulation using HPLC-PDA method. J App Pharm Sci, 2018; 8(07): 001-009.
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Quality evaluation of Dendrobium based on ultra-performance liquid chromatography (UPLC) and chemometrics
Triterpenoids and phytosteroids from stem bark of Crataeva nurvala buch ham.
Shumaia Parvin, Md. Abdul Kader, Md. Abdul Muhit, Md. Ekramul Haque, Md. Ashik Mosaddik, Mir Imam Ibne Wahed