Research Article | Volume: 8, Issue: 7, July, 2018

Antibacterial and cytotoxicity activities of phenylbutanoids from Zingiber cassumunar Roxb.

Thongchai Taechowisan Savoranee Suttichokthanakorn Waya S. Phutdhawong   

Open Access   

Published:  Jul 30, 2018

DOI: 10.7324/JAPS.2018.8719
Abstract

Bioautography was employed as the screening method for purifying bioactive substances of the crude extract of Zingiber cassumunar Roxb. Purification procedures included silica gel 60 column chromatography, thin layer chromatography, and medium pressure liquid chromatography. Identification of purified compounds was achieved by spectroscopic methods. Three phenylbutanoids were purified and identified as (E)-3-(3,4-dimethoxyphenyl)- 4-[(E)-3,4-dimethoxystyryl] cyclohex-1-ene (1), (E)-4-(3,4-dimethoxyphenyl)-but-3-en-1-ol (2) and (E)-4-(3,4- dimethoxyphenyl)-but-3-en-1-yl acetate (3). Compound 1 showed high antibacterial activity against Staphylococcus aureus and Escherichia coli with both MIC (16 μg/ml) and MBC (32 μg/ml). These were followed by the MIC values (32 μg/ml) and MBC values (128 μg/ml) for compounds 2 and 3 against the same microorganisms. These compounds revealed bacteriolytic effects on the assayed strains, causing evident damage to cell walls and membranes using SYTOX Green. The cytotoxicity activity of purified compounds was determined using MTT colorimetric assay against L929 and Vero cell lines. They showed weak cytotoxicity activity with IC50 values of 1263.42 to 2857.83 μg/ ml and 1537.83 to 2698.45 μg/ml toward L929 and Vero cell lines, respectively.


Keyword:     Antibacterial activity cytotoxicity activity phenylbutanoids Plai Zingiber cassumunar Roxb.


Citation:

Taechowisan T, Suttichokthanakorn S, Phutdhawong WS. Antibacterial and cytotoxicity activities of phenylbutanoids from Zingiber cassumunar Roxb. J App Pharm Sci, 2018; 8(07): 121- 127.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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