Research Article | Volume: 7, Issue: 11, November, 2017

Berberine mitigates methotrexate-induced oxidative stress and inflammation in the cerebrum of rats

Walaa G. Hozayen Shimaa M. Ramadan Abdulmannan Fadel Ayman M. Mahmoud   

Open Access   

Published:  Nov 30, 2017

DOI: 10.7324/JAPS.2017.71106

Berberine is a natural isoquinoline alkaloid with multiple beneficial therapeutic effects. This study was designed to evaluate the protective effect of berberine against methotrexate (MTX)-induced oxidative stress and inflammation in the brain of rats. Rats received a single intraperitoneal injection of MTX (20 mg/kg) and orally administered 25 mg/kg and 50 mg/kg body weight berberine for 7 days. MTX-induced rats showed significantly increased lipid peroxidation and nitric oxide levels in the cerebrum. Treatment of the MTX-induced rats with berberine produced a significant decrease in cerebral levels of lipid peroxidation and nitric oxide. In addition, berberine induced a significant increase in reduced glutathione, superoxide dismutase, glutathione peroxidase and glutathione-S-transferase in the cerebrum of MTX-induced rats. Rats received MTX showed a significant up-regulation of nuclear factor-kappaB (NF-κB), inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α) and interleukin-1beta (IL-1β) expression in the cerebrum, an effect that was significantly reversed following treatment with berberine. In conclusion, berberine protects against MTX-induced neurotoxicity through attenuating oxidative stress and inflammation, and boosting the antioxidant defenses.

Keyword:     Methotrexate Inflammation Berberine Oxidative stress.


Hozayen WG, Ramadan SM, Fadel A, Mahmoud AM. Berberine mitigates methotrexate-induced oxidative stress and inflammation in the cerebrum of rats. J App Pharm Sci, 2017; 7 (11): 043-049.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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