Research Article | Volume: 7, Issue: 7, July, 2017

Preliminary safety assessment of Eudragit® polymers nanoparticles administration in the rat brain

Mosaad A. Abdel-Wahhab,  Olivier Joubert,  Yasser A. Khadrawy,  Ramia Safar ,  ,  Aziza A. El-Nekeety,  Carole Ronzani,  Hussein G. Sawie,  Nabila S. Hassan,  Luc Ferrari,  Bertrand H. Rihn   
Mosaad A. Abdel-Wahhab1, Olivier Joubert2, Yasser A. Khadrawy3, Ramia Safar2 , 5, Aziza A. El-Nekeety1, Carole Ronzani2, Hussein G. Sawie3, Nabila S. Hassan4, Luc Ferrari2, Bertrand H. Rihn2

1Food Toxicology & Contaminants Department, National Research Center, Dokki, Cairo, Egypt.

2Faculty of Pharmacy, EA 3452 CITHEFOR, Lorraine University, 54001 Nancy Cedex, France.

3Medical Physiology Department, National Research Center, Dokki, Cairo, Egypt.

4Pathology Department, National Research Center, Dokki, Cairo, Egypt.

5Faculty of Pharmacy, AL-Baath University, Homs, Syria.

Open Access   

Published:  Jul 30, 2017

DOI: 10.7324/JAPS.2017.70726
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Abstract

Although nanotechnology can provide useful carriers to protect successfully active agents, it is necessary to determine the possible toxicity of these technological products before any extensive use. The aim of the current study was to evaluate the effects on the nervous system of Eudragit® polymers nanoparticles (EUD-Nps), considered as model of drug delivery carriers. Nile red-labeled EUD-NPs were prepared by a double emulsion/solvent evaporation technique. Male Sprague-Dawley rats were treated orally (PO) or intraperitoneally (IP) with a single dose of EUD-NPs (50 mg/kg bw). Animals were sacrificed after 4h, 48h, 1 week and 3 weeks and the brain of each rat was removed and dissected into the forebrain, midbrain and hindbrain regions. Acetylcholinesterase (AChE) activity, lipid peroxidation, reduced glutathione and nitric oxide were determined in different brain region homogenate. Other samples were used for the histological and fluorescent examinations. The results indicated that within the two routes of administration, all the tested parameters showed insignificant changes during the tested time intervals and all of them were in the normal range of the control by the 3rd week. Moreover, the histological examination revealed a normal histological picture of the brain tissues after 3 weeks of administration. The fluorescent examination showed that EUD-NPs were found in the brain tissues at all the studied times. These findings showed that EUD-NPs have the ability to cross the blood brain barrier but induced little toxic effects on the brain. Further studies are needed to verify the fate of these NPs in the long term before using them as a vector.


Keyword:     Eudragit® polymers nanoparticles toxicity brain oxidative stressacetylcholiesterase activity Sprague-Dawley rats.

Citation:

Abdel-Wahhab MA, Joubert O, Khadrawy YA, Safar R, ElNekeety AA, Ronzani C, Sawie HG, Hassan NS, Ferrari L Rihn RH. Preliminary safety assessment of Eudragit® polymers nanoparticles administration in the rat brain. J App Pharm Sci, 2017; 7 (07): 176-185.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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