Research Article | Volume: 6, Issue: 12, December, 2016

Determination of Estragole in Pharmaceutical Products, Herbal Teas and Herbal Extracts Using GC-FID

Omnia A. Ismaiel Essam Abdelghani Heba Mousa Samih I.Eldahmy B. E. Bayoumy   

Open Access   

Published:  Dec 28, 2016

DOI: 10.7324/JAPS.2016.601220

Recent concerns about the potential carcinogenicity of estragole necessitate accurate determination of estragole in different products and extracts. GC-MS method has been used for characterization of Fennel extract; Estragole was found to constitute more than 65% of the extract. Accurate and simple method for the determination of estragole in pharmaceutical products, herbal teas and herbal extracts was developed using GC-FID technique and p-anisaldehyde as an internal standard. Target analyte was extracted from different matrices by applying various extraction procedures such as hydro-distillation and ultrasound-assisted extraction. The hydro-distillation technique provided higher amounts of extracted estragole compared to ultrasound-assisted extraction. The calibration curve showed excellent linearity over a concentration range of 0.1–10 mg/ml with a correlation coefficient of 0.9997. Accuracy of back calculated calibration standards were within ±7.3 %. Precision and accuracy of quality control samples were within ± 9.0 %. Estragole levels were accurately measured in Fennel fruits, Chinese and Japanese Star Anise, Sekem® teabags for cough, Baby Calm® teabags, Balsam®, Guava® syrup for cough and Aqua ream® syrup for diarrhea. Accurate concentrations of estragole should replace the non-specific label information found on most of the tested products.

Keyword:     Estragole fennel star anise hydro-distillation teabags and pharmaceutical products.


Ismaiel OA, Abdelghani E, Mousa H, Eldahmy SI, Bayoumy BE. Determination of Estragole in Pharmaceutical Products, Herbal Teas and Herbal Extracts Using GC-FID. J App Pharm Sci, 2016; 6 (12): 144-150.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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