Research Article | Volume: 6, Issue: 12, December, 2016

Simple Spectrophotometric and Conductometric Methods for Determination of Gemifloxacin in Pure, Pharmaceutical Dosage Form and Human Urine

Dalia Zidan Omnia A. Ismaiel Wafaa S. Hassan Abdalla Shalaby   

Open Access   

Published:  Dec 28, 2016

DOI: 10.7324/JAPS.2016.601219
Abstract

Two Simple, accurate, precise, and rapid spectrophotometric and conductometric methods were developed for the estimation of gemifloxacin (GEM) in pharmaceutical dosage forms and biological human urine. Method A: A spectrophotometric method is based on the oxidation reaction between phosphomolybdic acid (PMA) and GEM to form molybdenum blue (Mo+5). Beer,s law was obeyed in the concentration range of (5-27 µg/ml). The correlation coefficient (𝑟2) for the studied drug was found to be 0.9999. The molar absorptivity (𝜀), Sandell’s sensitivity, limit of detection (LOD), and limit of quantitation (LOQ)were also calculated. Method B: A conductometric method is based on formation of an ion associate with PMA. It involves direct titration with PMA in the range of 1-20 mg. The precipitate obtained by ion pairing GEM with PMA has been spectroscopically characterized using IR spectroscopy. The method was successively applied to pharmaceutical formulations containing GEM. The results obtained were favorably compared with those obtained using the reported method.


Keyword:     Gemifloxacin Phosphomolybdic Acid Pharmaceutical Preparations Human Urine Spectrophotometry Conductometry IR spectroscopy.


Citation:

Zidan D., Ismaiel OA, Hassan WS, Shalaby A. Simple Spectrophotometric,Conductometric and Spectroscopic Methods for Determination of Gemifloxacin in Pure, Pharmaceutical Dosage Form and Human Urine. J App Pharm Sci, 2016; 6 (12): 136-143.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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