Research Article | Volume: 6, Issue: 4, April, 2016

Prevalence of Pfmdr 1 N86Y and Y184F Alleles is Associated with Recurrent Parasitemia following Treatment of Uncomplicated Malaria with Artemether-Lumefantrine in Nigerian Patients

Ayogu Ebere Emiliaa b Ukwe Chinwe Victoriab Mgbeahuruike Anthony Christianc Nna Emmanuel Okechukwud   

Open Access   

Published:  Apr 30, 2016

DOI: 10.7324/JAPS.2016.60403
Abstract

We investigated and compared genetic variations in Plasmodium falciparum multidrug resistance 1 gene (Pfmdr 1) in patients showing good therapeutic response (GTR) and artemisinin resistance (AR) following artemether-lumefantrine (AL) treatment of uncomplicated malaria in Nigeria. Some 150 malaria patients were subjected to AL treatment and therapeutic efficacy was monitored for 28 days. Parasite genomic DNA was isolated followed by nested polymerase chain reaction (PCR). Genotyping of Pfmdr 1 gene for specific genetic variants: N86Y, Y184F, S1034C and N1042D were done using PCR-restriction fragment length polymorphism (PCR-RFLP). Out of 121 patients that were P. falciparum positive, 46 % (56) and 54 % (65) showed good therapeutic response and artemisinin resistance respectively, with 5.4 % and 98.3 % being mutated in the GTR and AR group respectively. The most prevalent mutations were Y184F (44.1 %) and N86Y (40.7 %). There was significant increase (p<0.001) in the prevalence of Pfmdr 1 mutation in the post treatment compared to the pre-treatment group. Prevalence of Pfmdr1 86Y and 184F alleles is associated with artemisinin resistance and presence of AL drug significantly induced genetic variation in the plasmodial gene.


Keyword:     Plasmodium falciparum artemether-lumefantrine Pfmdr 1 gene genotyping.


Citation:

Ayogu EE, Ukwe CV, Mgbeahurike AC, Nna EO. Prevalence of Pfmdr1 86y and 184f Alleles is Associated with Recurrent Parasitemia Following Treatment of Uncomplicated Malaria with Artemether-Lumefantrine in Nigerian Patients. J App Pharm Sci, 2016; 6 (04): 015-021.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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