Research Article | Volume: 6, Issue: 1, January, 2016

Spectrophotometric Determination of Gentamicin Loaded PLGA Microparticles and Method Validation via Ninhydrin-Gentamicin Complex as a Rapid Quantification Approach

Ahmad Fahmi Harun Ismail,  Farahidah Mohamed,  Luqman Muizzuddin Mohd Rosli,  Mohd Affendi Mohd Shafri,  Muhammad Salahuddin Haris,  Anugerah Budipratama Adina   
Ahmad Fahmi Harun Ismail1, Farahidah Mohamed1, Luqman Muizzuddin Mohd Rosli1, Mohd Affendi Mohd Shafri2, Muhammad Salahuddin Haris1, Anugerah Budipratama Adina2

1Kulliyyah of Pharmacy (KOP), International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, Malaysia.

2Kulliyyah of Allied Health Sciences (KAHS), International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, Malaysia.

Open Access   

Published:  Jan 26, 2016

DOI: 10.7324/JAPS.2016.600102
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Abstract

The purpose of this study was to provide a quantification method with rapid, sensitive, reproducible and cost effective for gentamicin in the form of ninhydrin-gentamicin complex. The utilization of spectrophotometric module to validate the method development for gentamicin loaded microparticles was intended to provide alternative quantification method without undermining the sensitivity of the developed method. The microparticles fabrication process was proven to be suitable in encapsulating gentamicin by using poly(lactic-co-glycolic acid) PLGA without compromising the efficacy of the antibiotic itself. The linearity of 6 known concentrations of ninhydrin-gentamicin complex was obtained with the R2 of 0.9998. The interday and intraday precisions were determined with the acceptance %RSD values of less than 2%. The highest %RSD value was 1.09% which suggested the method to be acceptably precise. The limit of detection (LOD) and limit of quantification (LOQ) values were recorded to be at 0.016 and 0.196 mg/mL respectively. The % recovery of 4 known concentrations indicated the accuracy of the method was high with the recovery range between 98.66% and 101.8%. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines. This quantification method was a promising approach to provide a rapid and cost effective in evaluating gentamicin concentration for in-vitro applications.


Keyword:     Spectrophotometry Gentamicin Ninhydrin Microparticles poly(lactic-co-glycolic acid).

Citation:

Ismail AFH, Mohamed F, Rosli LMM, Shafri MAM, Haris MS, Adina AB. Spectrophotometric Determination of Gentamicin Loaded PLGA Microparticles and Method Validation via Ninhydrin-Gentamicin Complex as a Rapid Quantification Approach. J App Pharm Sci, 2016; 6 (01): 007-014.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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