Research Article | Volume: 5, Issue: 4, April, 2015

Spectrophotometric determination of acyclovir after its reaction with ninhydrin and ascorbic acid

Ukpe Ajima Johnson Ogoda Onah   

Open Access   

Published:  Apr 27, 2015

DOI: 10.7324/JAPS.2015.50411

Acyclovir is a purine-based nucleoside antiviral agent used in the management of Herpes simplex and other viral infections. The present study is aimed at developing and validating a simple and rapid spectrophotometric method for its determination. The mechanism of the proposed method is based on the condensation/coupling reaction between Acyclovir and Ninhydrin-Ascorbic acid at pH 5. A purple colored product with maximum absorption at 540 nm was assayed to quantitatively evaluate the drug content in the formulation. The calibration curve was found to be linear up to 30 µg/ml. Analyte recovery tests carried out by the proposed method gave recovery of between 96.9 – 102.0%. Molar absorptivity and Sandells’ sensitivity were determined to be 41,071.43 L mol-1 cm-1 and 1.84 µg cm-2 respectively. The precision was assessed by determining the inter-day and intra-day variation which ranged between 1.45 – 1.63 % and 0.81 – 1.12 % respectively. The results show that the reaction produced a stable product and the proposed method is cost-effective and possesses adequate accuracy, precision and sensitivity. It can therefore be conveniently applied for the determination of acyclovir in dosage forms.

Keyword:     Acyclovir Ninhydrin Ascorbic acid Spectrophotometry Pharmaceutical analysis.


Ajima U., Onah J. O. Spectrophotometric Determination of Acyclovir After its Reaction With Ninhydrin and Ascorbic Acid. J App Pharm Sci, 2015; 5 (04): 065-069.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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