Evaluation on the Bacillus subtilis spore in drug delivery

Bacillus subtilis spores were used to study on drug delivery nowadays. However, evaluation on the cisplatin packaging and releasing ability in spores is still limited. In this study, we determined Bacillus subtilis spores (2x108) could be packaged to 54,600,000 drug molecules of cisplatin from 8 to 12 hours. Spores were checked by scanning electron microscope and transmission electron microscope, and then drug release was done. Cisplatin started releasing at 0.5 hours at pH 3.4 (23.8%) that was higher than pH 7.2 (0.5%). Cisplatin released irregularly during 24 hours in pH 3.4. In contrast, the cisplatin release was slow and steady during 24 hours in pH 7.2. The release ability was only 25% that meant that cisplatin packing spores would be used in prolonged-release finished products. The study was the first report of the cisplatin-packaged spore and release ability of cisplatin out of spore that could be used in pharmaceutical field.