The effects of Hydnophytum formicarum ethanolic extract towards lymphocyte, vero and T47d cells proliferation in vitro

This research aims were to explore the effects of Hydnophytum formicarum Jack (Rubiaceae) known as the Ant plant on proliferations of mouse lymphocyte, Vero and T47D's cell lines. Plant hypocotyls were collected from West Papua. Some parts were dried to gain plant with ant residue (A), while other parts were separated from the ant residue to gain plant without ant residue (B) and ant residue (C). After being macerated in ethanol and evaporated to dryness, each extract was tested on Balb/c mice lymphocytes and Vero cell lines by MTT assay in the presence and absence of Doxorubicin (Dox). Effect on T47D was observed by using Dox 25 µg/mL as control. Data obtained were analyzed by ANOVA and paired sample T-test. Thin layer chromatography profiles were evaluated to identify the difference in chemical contents. The extracts A and B had the ability to increase lymphocyte proliferation by increasing concentration. Both extracts could not significantly enhanced the proliferation of Vero cells, neither in the presence nor absence of Dox. The extract B in 100 µg/mL showed the highest inhibition against T47D cells by 53.45%. There was no chemical content difference observed on extracts A and B.