Clausena suffruticosa (Family: Rutaceae) root ethanolic extract was subjected to analyze for its antioxidant effect by DPPH free radical scavenging method, antibacterial & antifungal effect by disc diffusion technique and cytotoxic effect by brine shrimp lethality test. The extract showed very significant antioxidant activity with the LC50 value of 3.28µg/ml. In antibacterial screening, moderate zone of inhibition (7.5-9.0mm in diameter) was observed against gram positive Bacillus subtilis, Bacillus cereus, Streptococcus aureus, Bacillus polymyxa & Bacillus megaterium and promising zone of inhibition (10.0-13.0mm in diameter) against gram negative Salmonella typhi, Shigella flexneri,, Proteus sp. & Escherichia coli. Klebsiella sp. and Shigella sonnei did not show sensitivity. The MIC values against these bacteria were ranged from 3,000 to 4,500µg/ml. Root extract showed significant zone of inhibition against Aspergillus ustus, Aspergillus niger and Aspergillus ochraceus in antifungal assay. In the cytotoxicity assay, LD50 and Chi-square value of the ethanolic extract against brine shrimp nauplii were 546.94µg/ml and 1.9932 demonstrating potent cytotoxic effect of the extract.
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