Research Article | Volume: 4, Issue: 6, June, 2014

Cytotoxic activity and apoptosis induction of 8-hydroxyisocapnolactone-2´,3´-diol and its combination with Doxorubicin on MCF-7 and T47D cells

Ratna Asmah Susidarti Riris Istighfari Jenie Muthi’ Ikawati Dyaningtyas Dewi Pamungkas Putri Edy Meiyanto   

Open Access   

Published:  Jun 28, 2014

DOI: 10.7324/JAPS.2014.40614
Abstract

The cytotoxic activity and apoptosis induction of 8-hydroxyisocapnolactone-2΄,3΄-diol (HICD) and its combination with doxorubicin (Doxo) on MCF-7 and T47D cells have been evaluated. The cytotoxic assay was performed using MTT method. The IC50 was used to express the cytotoxic potency, while the combination index (CI) was calculated to determine the effect of combination. The apoptotic assay was carried out using acrydine orange - ethidium bromide double staining method, while Bcl-2 and Bax expression were investigated by immunocytochemistry. The HICD exhibited cytotoxic activity on MCF-7 and T47D cells with the IC50 of 8 µg/ml (21.2 µM) and 4 µg/ml (10.6 µM), respectively. The combination of HICD with Doxo showed synergistic effect and increased apoptosis induction on both cell lines. The HICD did not affect Bcl-2 but increased Bax expression on MCF-7 cells, while on T47D cells it suppressed Bcl-2 expression but did not modulate Bax expression


Keyword:     8-Hydroxyisocapnolactone-2'3'-diol Cytotoxic activity Apoptosis induction Doxorubicin MCF-7 T47D.


Citation:

Ratna Asmah Susidarti, Riris Istighfari Jenie, Muthi’ Ikawati, Dyaningtyas Dewi Pamungkas Putri, Edy Meiyanto. Cytotoxic activity and apoptosis induction of 8-hydroxyisocapnolactone-2ï‚¢,3ï‚¢- diol and its combination with Doxorubicin on MCF-7 and T47D cells. J App Pharm Sci, 2014; 4 (06): 089-097.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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