Research Article | Volume: 4, Issue: 4, April, 2014

Cytotoxicity assessment of aqueous extract from root barks of Calotropis procera (Ait.) R. Br. in human intestinal Caco-2 and mouse neuroblastoma Neuro-2a cell lines

Geoffroy G. Ouedraogo ,  ,  Serge Moukha,  Théophile A. Mobio,  Moustapha Ouedraogo,  Pierre I. Guissou ,  and Edmond E. Creppy   
Geoffroy G. Ouedraogo1 , 2, Serge Moukha2, Théophile A. Mobio2, Moustapha Ouedraogo1, Pierre I. Guissou1 , 3 and Edmond E. Creppy2

1Laboratoire de Toxicologie, Environnement et Santé ; Ecole Doctorale de la Santé, Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso,

2Laboratoire de Toxicologie et d’Hygiène Appliquée, UFR des sciences pharmaceutiques, Université Bordeaux-2, 146 rue Leo Saignat, 33076 Bordeaux, France,

3Institut de Recherche en Science de la Santé / Centre National de Recherche Scientifique et Technologique (IRSS/CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso.

Open Access   

Published:  Apr 28, 2014

DOI: 10.7324/JAPS.2014.40401
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Abstract

Calotropis procera (Ait.) R. Br (Asclepiadaceae) is a species widely used in traditional medicine for the treatment of various diseases such as sickle cell disease, asthma and cancer. In Burkina Faso, it enter in the composition of FACA® in combination with Zanthoxylum zanthoxyloides Lam (Rutaceae), drug used in sickle cell disease treatment. The objective of this study was to evaluate the in vitro cytotoxicity of aqueous extract of root barks of the plant on cell lines to increase the safe use of FACA®. MTT and Neutral Red assays performed on Caco-2 and Neuro-2a cell lines revealed that aqueous extract from root barks of Calotropis procera are cytotoxic on these cell lines. DNA fragmentation assay on Caco-2 cell showed DNA smearing reflecting a degradation of nuclear material that indicates a possible genotoxicity. Altogether, it comes out that the most sensitive cell line is the human colorectal carcinoma Caco-2 cells. Comparatively the active compounds of Calotropis procera do not affect the mice nervous system cells in the same dramatic extent. Our results strongly suggest that patients under treatment of FACA® must respect doses prescribed in order to avoid adverse side effects on the gastrointestinal tract.


Keyword:     Calotropis procera Ait. cytotoxicity FACA® Caco-2 cell Neuro-2a cell.

Citation:

Geoffroy G. Ouedraogo, Serge Moukha; Théophile A. Mobio, Moustapha Ouedraogo, Pierre I. Guissou and Edmond E. Creppy.,Cytotoxicity assessment of aqueous extract from root barks of Calotropis procera (Ait.) R. Br. in human intestinal Caco-2 and mouse neuroblastoma Neuro-2a cell lines. J App Pharm Sci, 2014; 4 (04): 001-007.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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