Research Article | Volume: 4, Issue: 3, March, 2014

In vitro evaluation of antimicrobial, cytotoxic and antioxidant activities of Crude methanolic extract and other fractions of Sterculia villosa barks

Syed Sami-ul Haque Md. Mamun Or Rashid Md. Akhtaruzzaman Prodhan Sadia Noor Atanu Das   

Open Access   

Published:  Feb 27, 2014

DOI: 10.7324/JAPS.2014.40308
Abstract

Crude methanolic extract and other fractions of Sterculia villosa barks were investigated for their antimicrobial, cytotoxic and antioxidant activity in this study. Antimicrobial activity of different extract was evaluated by measuring the diameter of the zone of inhibition against gram-positive & gram-negative bacteria and fungi using Ciprofloxacin as a standard antimicrobial agent. Free radical scavenging activity for the same extracts was determined by DPPH assay where BHT was used as positive control and Cytotoxicity was determined by Brine Shrimp nauplii where the minimum inhibitory concentration was assessed by serial dilution technique. Mild antimicrobial activity was found; crude methanolic extract showed effect against all the organisms, while other extracts showed effect for some of the organisms. The LC50 value for cytotoxicity assay was found 0.3, 2.95, 3.76, 35.33 & 55.98 µg/ml for CSV, PESV, CTSV, DCMSV & EASV extracts respectively where LC50 value of Vincristine Sulfate was 0.544 µg/ml. Ethyl acetate fraction showed good antioxidant properties and except Pet Ether fraction all other extracts showed considerable antioxidant activity. The bark of Sterculia villosa can be considered for further research for finding potent compounds of antioxidant, antimicrobial and cytotoxic activity.


Keyword:     Sterculia villosa bark antimicrobial cytotoxic antioxidant DPPH assay Brine shrimp lethality bioassay.


Citation:

Syed Sami-ul Haque, Md. Mamun Or Rashid, Md. Akhtaruzzaman Prodhan, Sadia Noor, Atanu Das. In-vitro evaluation of antimicrobial, cytotoxic and antioxidant activities of Crude methanolic extract and other fractions of Sterculia villosa barks. J App Pharm Sci, 2014; 4 (03): 035-040.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
1023 Absract views 119 Absract Downloads 1142 Total
Related Search

By author names