Evaluation of phytoconstituents, nephro-protective and antioxidant activities of Clitoria ternatea

Clitoria ternatea(CT), is an herbaceous medicinal plant used to treat, liver problems India. Acetaminophen is a commonly used analgesic and antipyretic agent which, at high doses, causes liver and kidney necrosis in man and animals. The aim of the present study is to evaluate phytoconstituents and investigate the nephroprotective & antioxidant activities of the ethanol extract of Clitoria ternatea on acetaminophen induced toxicity in rats. Phytoconstituents like 1H-Cycloprop[e]azulene,1a,2,3,5,6,7,7a,7b-octahydro-1,1,4,7-tetramethyl-,[1aR- (1aà,7à,7aá,7bà)]- [Synonyms: Varidiflorene], Pterocarpin, 6H-Benzofuro[3,2-c][1]benzopyran, 6a,11a-dihydro-3,9-dimethoxy-, (6aR-cis)- [Synonyms: Homopterocarpin], Isoparvifuran, Hexadecanoic acid, ethyl ester, Myo-Inositol, 4-C-methyl-, 1,2,3,5-Cyclohexanetetrol, (1à,2á,3à,5á)-, Propane, 1,1-diethoxy- were identified from ethanol extract of Clitoria ternatea by using a gas chromatograph-mass spectrograph (GC MS). Biochemical studies show that there is an increase in the levels of serum urea and creatinine along with an increase in the body weight and reduction in the levels of uric acid in acetaminophen induced groups. These values are retrieved significantly by treatment with Clitoria ternatea extracts at two different doses. The antioxidant studies reveal that the levels of renal SOD, CAT, GSH and GPx in the APAP treated animals are increased significantly along with a reduced MDA content in ethanol extract of Clitoria ternatea treated groups. Apart from these, histopathological changes also reveal the protective nature of the Clitoria ternatea extract against acetaminophen induced necrotic damage of renal tissues. In conclusion, these data suggest that the ethanol extract of Clitoria ternatea can prevent renal damage from APAP induced nephrotoxicity in rats and it is likely to be mediated through active phytoconstituents and its antioxidant activities.