An ion selective membrane sensor from dioctyl phthalate as a plasticizer in a polymeric matrix of polyvinyl chloride (PVC) and β-cyclodextrin as an ionophore was constructed and evaluated according to IUPAC recommendations. Linear Nernestian response of 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH•) within the concentration ranges of 10−6 to 10−2 mol L−1 was obtained with average recovery 99.87±0.617. Nernstian slope of 58.5 mV/decade with excellent selectivity over the pH range of 3–8 was observed. The suggested method was standardized using butylated hydroxyl anisole (BHA). The 50% radical scavenging activity (IC50) determined by the proposed sensor correlated well with that of the common colorimetric method based on scavenging of DPPH•. An algorithm implemented in Microsoft Visual Basic® 6.0 was used for calculating (IC50) values which are 7.38 μg/ml ± 0.35, 89.98 μg/ml ± 0.45 and 1.45 mg/ml± 1.50 for BHA, paracetamol and dipyridamole, respectively. The proposed sensor represents a simple and reproducible tool for measuring DPPH• scavenging activity of paracetamol and dipyridamole in bulk powder, pharmaceutical formulations and simulated intestinal fluid (SIF) without sophisticated separation techniques.
M. El-Kosasy, L. A. Hussien, M. H. Abdel-Rahman. New approach for measuring antioxidant activity via graphite sensor . J App Pharm Sci, 2013; 3 (09): 071-077.
Year
Month
Effects of substitution of pellet of Moringa oleifera to commercial feed on rabbit’s digestion, growth performance and carcass trait
Evaluation of phytoconstituents, nephro-protective and antioxidant activities of Clitoria ternatea
K.Sarumathy, M.S.Dhana Rajan, T.Vijay, J.JayakanthiEffects of Phosphate Modified and Pregelatinized Sweet Potato Starches on Disintegrant Property of Paracetamol Tablet Formulations
I. Jubril, J. Muazu, G. T. Mohammed