Research Article | Volume: 3, Issue: 8, August, 2013

Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity

Suresh P. Kamble Rajendra B. Gaikar Rimal B. Padalia and Keshav D. Shinde   

Open Access   

Published:  Aug 30, 2013

DOI: 10.7324/JAPS.2013.3826
Abstract

C-Phycocyanin (C-PC) is a phycobiliprotein found in blue green algae, such as Spirulina platensis, is often used as a dietary nutritional supplement and exhibit a variety of pharmacological properties. In this regard, extraction, partial purification and antioxidant, anticoagulation and prevention of DNA damage activity of C-PC was investigated. In the present study, a simple and efficient method to extract C-PC from Spirulina platensis dry powder is reported. The extractions were carried out using two different methods: cold maceration and sonication method. The extraction using cold maceration method proved to be the most efficient method. Obtained crud C-PC was purified by ammonium sulphate precipitation, dialysis and gel filtration and presented a final extraction yield of 3.27±0.09 mg/ml with a purity ratio of 2.317±0.08. When it was evaluated as an antioxidant in vitro, it was able to scavenge nitric oxide. C-PC showed significant anticoagulation and prevention of DNA damage activity.


Keyword:     Spirulina Extraction C-phycocyanin DNA damage SDS-PAGE Antioxidant activity Anticoagulation activity.


Citation:

Suresh P. Kamble, Rajendra B. Gaikar, Rimal B. Padalia and Keshav D. Shinde., Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity. J App Pharm Sci, 2013; 3 (08): 149-153.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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