Research Article | Volume: 3, Issue: 6, June, 2013

Atrazine degradation in liquid culture and soil by a novel yeast Pichia kudriavzevii strain Atz-EN-01 and its potential application for bioremediation

Evy Alice Abigail Jaseetha Abdul Salam and Nilanjana Das   

Open Access   

Published:  Jun 27, 2013

DOI: 10.7324/JAPS.2013.3606
Abstract

A novel yeast strain, Atz-EN-01 isolated from contaminated-agricultural soil was found to be highly effective in degrading atrazine in liquid culture and soil. The molecular characterization based upon partial 18S rDNA and ITS regions identified the strain Atz-EN-01 as Pichia kudriavzevii. The yeast could degrade atrazine completely within 7 days with a rate constant of 0.31 per day following the first order kinetic model. The time in which initial atrazine concentration (500 mg/L) was reduced by 50% (half-life) was 2.2 days under optimal conditions (pH 7.0, temperature 30Ëš C, inoculum size 3% (v/v) and shaking speed 120 rpm). The analysis of the metabolites using GC-MS identified the formation of 3 intermediates viz. hydroxyatrazine, N-isopropylammelide and cyanuric acid. The enzyme atrazine chlorohydrolase exhibited maximum activity during degradation. Based upon the intermediates identified by GC-MS and FT-IR analysis, the sequential process of atrazine degradation was proposed. In soil bioremediation experiment, inoculation of soil with Atz-EN-01 promoted effective degradation than did the control. To the best of our knowledge, this is the first report on Pichia kudriavzevii strain Atz-EN-01 which can serve as a potential agent for in-situ bioremediation of atrazine contaminated environment.


Keyword:     Atrazine Biodegradation Kinetics Pichia sp. Soil.


Citation:

Evy Alice Abigail, Jaseetha Abdul Salam and Nilanjana Das., Atrazine degradation in liquid culture and soil by a novel yeast Pichia kudriavzevii strain Atz-EN-01 and its potential application for bioremediation. J App Pharm Sci, 2013; 3 (06): 035-043.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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