Research Article | Volume: 3, Issue: 4, April, 2013

Induction of Apoptosis on MCF-7 cells by Selaginella Fractions

Sri Handayani Adam Hermawan Edy Meiyanto Zalinar Udin   

Open Access   

Published:  Apr 27, 2013

DOI: 10.7324/JAPS.2013.3405
Abstract

The selaginella ethanolic extract shows cytotoxic activity against T47D and MCF-7 cells. The aim of this research is to evaluate the cytotoxic effect and apoptosis induction of selaginella fractions on MCF-7 cells. The Selaginella plana powder was extracted by absolute ethanol. Ethanolic extract was dilluted by methanol : water (4:1) and then fractionated by hexane (S_Hex), methylene chloride (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). Cytotoxic activity was examined by MTT assay. Apoptosis examination used acrydine orange-etidium bromide staining (double staining). The result showed that the IC50 value of S_Hex, S_MTC, S_EA, and S_BuOH on MCF-7 cells were 30 µg/mL, 19 µg/mL, 24 µg/mL, and 2 µg/mL respectively. The active fractions (S_Hex, S_MTC, S_EA and S_BuOH) at its IC50 concentration increased apoptotic cells on the MCF-7 cells 35.33%, 20.33%, 24% and 45.67% respectively compared to control. Based on the result, buthanol fraction of Selaginella plana (S_BuOH) showed the highest apoptotic induction on MCF-7 cancer cells.


Keyword:     Selaginella plana Hieron MCF-7


Citation:

Sri Handayani, Adam Hermawan, Edy Meiyanto, Zalinar Udin., Induction of Apoptosis on Mcf-7 cells by Selaginella Fractions. J App Pharm Sci, 2013; 3 (04): 031-034.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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