Research Article | Volume: 3, Issue: 2, February, 2013

Studies on Novel bacteriocin like inhibitory substance (BLIS) from microalgal symbiotic Vibrio spp MMB2 and its activity against aquatic bacterial pathogens

Selvendran M and Michael Babu M.   

Open Access   

Published:  Feb 27, 2013

DOI: 10.7324/JAPS.2013.30230
Abstract

The present study was aimed to isolate the antagonistic bacteria from marine microalgal sample (Chlorella salina), the isolated bacterial strains were identified, screened for their bioactivity against the aquatic pathogens, to extract and characterize the compounds produced by antagonistic bacteria. Among the isolates Vibrio sp.MMB2 strain and Pseudomonas sp. had good antagonistic property against all tested pathogens. The maximum biogenic activity was occurred at the stationary phase culture. The metabolites extracted from Vibrio sp. MMB2 was showed highest zone of inhibition against A.hydrophylla. One active fraction was isolated by TLC. The functional group was identified through FTIR analysis, the bacterial metabolites contains Nitrogenous compound as a functional groups. The Partially purified CFNS (bacteriocin) of Vibrio sp. MMB2 was showed two distinct bands on SDS-PAGE, its high molecular weight of antimicrobial peptides. The bacterial metabolites effectively controlled the pathogens at broad range, to compare commercial antibiotics.


Keyword:     Antagonistic bacteria Biogenic activity metabolites CFNS (Cell free Neutralized Supernatants).


Citation:

Selvendran M, and Michael Babu M., Studies on Novel bacteriocin like inhibitory substance (BLIS) from microalgal symbiotic Vibrio spp MMB2 and its activity against aquatic bacterial pathogens. J App Pharm Sci. 2013; 3 (02): 169-175.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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