Research Article | Volume: 3, Issue: 2, February, 2013

The antioxidant and anticoagulant activities of polysaccharides isolated from the brown algae Dictyopteris polypodioides growing on the Lebanese coast

Nadine Karaki Carine Sebaaly Nathalie Chahine Tarek Faour Alexandre Zinchenko Samar Rachid and Hussein Kanaan   

Open Access   

Published:  Feb 27, 2013

DOI: 10.7324/JAPS.2013.30208
Abstract

In this study, we attempt to isolate the polysaccharides from the brown seaweed Dictyopteris polypodioides (Dp) growing on the Lebanese coast. The percentages of the main polysaccharide alginic acid were 4.6% in May and 6.25% in July. The extracted yields of Fucoidan, Laminaran and Mannuronan (FLM) and Mannuronan (M) alone were respectively 0.75% and 0.38% in May and 1.15% and 0.67% in July. We performed the infrared spectroscopy in order to reveal the functional groups of alginate and mannuronan. Moreover, a non-destructive technique ¹ H NMR was used to determine the structure of alginate and the distribution of guluronic (G) and mannuronic acid (M) in the chain, allowing us therefore to calculate the ratio M/G. The latter was 0.96 showing a strong ability of alginate to capture heavy metals. The analysis of trace elements showed great amounts of K, Si, Na and Mg in both seasons. Moreover, Dictyopteris appears to be rich in fatty acids (10 items) with palmitic and oleic acids as main fatty acids. Furthermore, the anticoagulant activity of polysaccharides was investigated by activated partial thromboplastin time (APTT) clotting assay and the antioxidant activity was studied by electrolysis method. It has been shown that the FLM and M fractions of Dictyopteris polypodioides have a significant antioxidant and anticoagulant activities. Further investigations are imperative in order to develop new molecules based on these polysaccharides to be used as drugs or for healthcare in general.


Keyword:     Antioxidant anticoagulant brown algae Dictyopteri


Citation:

N. Karaki, C. Sebaaly, N. Chahine, T. Faour, A. Zinchenko, S. Rachid, H. Kanaan, The antioxidant and anticoagulant activities of polysaccharides isolated from the brown algae Dictyopteris polypodioides growing on the Lebanese coast. J App Pharm Sci. 2013; 3 (02): 043-051.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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