Research Article | Volume: 2 Issue: 9, September, 2012

Bioassay Guided Phytometabolites Extraction for Screening of Potent Antimicrobials in Passiflora foetida L.

Patil A.S. and Paikrao H.M   

Open Access    DOI: 10.7324/JAPS.2012.2927

Abstract

Passiflora is a genus belonging to the family Passifloraceae having varied species with highly therapeutic values. Tribes with traditional medicinal knowledge suggested Passiflora foetida L. as a source of high value pharmaceutical plant. Present study deals with the Isolation, Purification, characterization and bioassays of antimicrobial secondary metabolites. In spite of traditional soxhlet extraction, Cold percolation proved suitable extraction scheme. Bioassay guided TLC characterization, and purification led the effective collection of bioactive natural products. Disc diffusion method shows a potent inhibitory activity of compounds against multi drug resistant pathogenic microorganisms like S. aureus (OARS). For structural characterization bioactive products were analyzed using UV. The results confirmed the presence of polyacetylenes as active constituents in the plant.


Keyword:     Passiflora foetida L. Phytochemicals TLC-bioautography antimicrobial activity polyacetylenes.


Citation:

Patil A.S. and Paikrao H.M. Bioassay Guided Phytometabolites Extraction for Screening of Potent Antimicrobials in Passiflora foetida L. J App Pharm Sci. 2012; 2(9): 137-142.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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