Research Article | Volume: 2 Issue: 7, July 2012

Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Prednisolone and 5-Amino Salicylic Acid in Tablet Dosage form

Gurmeet Singh Dinesh Kumar Deepak Sharma Mankaran Singh Sukhbir Kaur   

Open Access    DOI: 10.7324/JAPS.2012.2736


The present work carries an analytical method development of prednisolone and 5-amino salicylic acid in tablet dosage form. The method is based upon Q – absorption ratio method for the simultaneous determination of the prednisolone and 5-amino salicylic acid. Both the drugs are widely used for bacterial cure and are recommended for the patients with mild to moderate inflammation of the digestive tract. Ulcerative colitis and crohn’s disease are the target disorders which are treated by both drug candidates. Absorption ratio method is used for the ratio of the absorption at two selected wavelength one of which is the iso-absorptive point and other being the λmax of one of the two components. Prednisolone and 5-ASA shows their iso-absorptive point at 283 nm in ethanol and 0.1N HCl respectively. The second wavelength used is 302 nm which is the λmax of 5-ASA in 0.1N HCl. The linearity was obtained in the concentration range of 1-10 µg/ml for prednisolone and 5-ASA. This method was applied to all pharmaceutical dosage form because there is no excipients interference between them. The results have been validated by recovery studies.

Keyword:     Prednisolone 5-ASA absorption ratio method linearity λmax.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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