Research Article | Volume: 2 Issue: 7, July 2012

Thrombolytic Activity and Preliminary Cytotoxicity of Five Different Fractions of Methanol Extract of Allamanda cathartica Leaf

Rehan Sarker Tasnuva Sharmin Sharmin Reza Chowdhury Farhana Islam   

Open Access    DOI: 10.7324/JAPS.2012.2717

Abstract

Investigation with the crude methanol extract of Allamanda cathartica leaves and its different fractions were carried out to evaluate its possible thrombolysis and cytotoxic activities. A quick & rapid methodology (In-vitro Thrombolytic model) was applied to find out their thrombolytic potential where streptokinase and water were employed as a positive and negative controls, respectively. Among the extractives, the chloroform (CSF) and hexane (HSF) soluble fractions showed 34.51±0.669% and 32.179±0.581% clot lysis activity respectively compared to standard streptokinase which exhibited 61.5% lysis of clot. In brine shrimp lethality bioassay method, the LC50 values of the test samples of A. cathartica leaves were assayed where DMSO and Vincristine sulphate were used as solvent & as positive control respectively. The chloroform, hexane and carbon tetrachloride soluble fractions showed significant cytotoxic activity against brine shrimp nauplii and LC50 values were 1.45, 5.00 and 5.24 μg/ml respectively.


Keyword:     Allamanda catharticaClot lysisIn-vitro Thrombolytic modelStreptokinaseCytotoxic activity.


Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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