Research Article | Volume: 6, Issue: 6, June, 2016

In vitro Anticoagulant Activity of Siddha Sastric Formulation Linga Mathirai compared with Low Molecular Weight Heparin

Thanigavelan Vembu Mathukumar Sivagnanam Pitchiah Kumar Murugan   

Open Access   

Published:  Jun 28, 2016

DOI: 10.7324/JAPS.2016.60611
Abstract

Linga Mathirai (LM) is a Siddha Sastric formulation indicated for treating cardiovascular diseases. The present study aimed to evaluate the efficacy of LM against the formation of thrombus and platelet aggregation on compared with Low molecular weight Heparin. In vitro clot lysis and platelet aggregation assays were employed for the study on blood samples of rat. Whole blood samples and platelets obtained by centrifugation were used for clot lysis and aggregation assay respectively. Platelet aggregation was induced by agonists Thrombin and Adenosine diphosphate (ADP). The different concentrations of LM (100, 200, 500, 1000 µL) were prepared with the adjuvant Ginger decoction for test samples and 100 µL low molecular weight heparin (LMWH) was used for treating standard group. Against clot formation and platelet aggregation, LM did not exhibit significant efficacy on compared with normal saline (control) and heparin. However, LM at its higher concentration 1000 µL exhibited significant inhibitory activity on platelet aggregation induced by both Thrombin and ADP when compared with distilled water (control). Under this study, it is concluded that the test drug Linga Mathirai had comparable and slightly lesser efficacy than low molecular weight heparin.


Keyword:     Siddha Mathirai Lingam Clot lysis Platelet aggregation Anticoagulant.


Citation:

Vembu T, Sivagnanam M, Murugan PK. In vitro Anticoagulant Activity of Siddha Sastric Formulation Linga Mathirai compared with Low Molecular Weight Heparin. J App Pharm Sci, 2016; 6 (06): 061-065.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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