Research Article | Volume : 1 Issue : 3, May 2011

Quantitative Estimation of Piperine in Piper nigrum and Piper longum Using High Performance Thin Layer Chromatography

P.D.Hamrapurkar Kavita Jadhav Sandip Zine   

Open Access   


Piperine, a characterizing compound present in fruits of Piper nigrum and Piper longum used as bioavailability enhancer. Ingredients of antioxidant, anti-inflammatory activity has been extracted using soxhlet and supercritical fluid extraction technique. It was isolated using column chromatography. Characterization of compound was done by spectroscopic technique. A simple, rapid, accurate and specific HPTLC method developed and validated. The method proposed can be used for the routine analysis of both P.nigrum and P.longum fruit material and its formulations.

Keyword:     HPTLC Fingerprinting Quantitative estimation Piper nigrum Piper longum Piperine.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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